Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Cloning of fiber-specific cDNAs and their structural variations in 4 fibermutants
Autore:
Wang, XD; Zhu, YX; Ji, DF; Jiang, SL; Li, YY;
Indirizzi:
Zhejiang Univ, Coll Agr & Biotechnol, Zhejiang 310029, Peoples R China Zhejiang Univ Zhejiang Peoples R China 310029 ng 310029, Peoples R China Beijing Univ, Coll Life Sci, Beijing 100871, Peoples R China Beijing UnivBeijing Peoples R China 100871 jing 100871, Peoples R China
Titolo Testata:
CHINESE SCIENCE BULLETIN
fascicolo: 3, volume: 46, anno: 2001,
pagine: 234 - 237
SICI:
1001-6538(200102)46:3<234:COFCAT>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACETOBACTER-XYLINUM; CELLULOSE SYNTHASE; COTTON; BIOSYNTHESIS; OPERON;
Keywords:
cotton; fiber mutant; fiber mRAN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
7
Recensione:
Indirizzi per estratti:
Indirizzo: Wang, XD Zhejiang Univ, Coll Agr & Biotechnol, Zhejiang 310029, Peoples R China Zhejiang Univ Zhejiang Peoples R China 310029 , Peoples R China
Citazione:
X.D. Wang et al., "Cloning of fiber-specific cDNAs and their structural variations in 4 fibermutants", CHIN SCI B, 46(3), 2001, pp. 234-237

Abstract

A mRNA preferentially expressed in cotton fiber was cloned from fiber total RNA of normal upland cotton TM-1 (wild-type) by using RT-PCR and corresponding cDNA (signed as TM-E6) was sequenced, TM-E6 gene had no intron and contained an open reading frame of 771 bp long, and might encode a peptide of246 amino acids, Other 4 genes, Fl-E6, Li-E6, N-E6 and Bl-E6, which were homologous to TM-E6 gene, were also isolated from 4 fiber mutants of Fiberless Xu-zhou 142, Ligon lintless, Naked seed and Brown lint, respectively. Sequence analysis of each of these mutant genes revealed many variations in structure and nucleotide composition of gene when compared with the sequenceof TM-E6 gene. (i) There was a changeable repetitive segment in which GGCTCA (Gly-Ser) was repeated 3-5 times between the 82nd and the 93rd codons indifferent mutant genes. Since the change of Gly-Ser repetitive segment occurred not only in the mutants but also in the wild-type cotton, the repeat frequency might not be associated with the mutation of fiber characteristics. (ii) Among the 4 mutant genes, the percentage of changed codons was 7.05% in Fl-E6, 4.98% in Li-E6, and 4.15% in N-E6 and Bl-E6. It seems that the percentage of changed codons in E6 sequence was positively correlated to the degree of fiber morphological variation. (iii) E6 polypeptides of two long-fiberless mutants (Fiberless Xuzhou 142 and Ligon lintless) contained high similar (99.4%) variation in the region of 1-174 amino acids from N-terminus, and those of short-fiberless mutants (Fiberless Xuzhou and naked seed)revealed identical variation in the region of 116th-220th amino acids. It also seems that there was a parallel relation between E6 protein variation and fiber phenotype mutation, (iv) Li-E6 and Bl-E6 genes also expressed at low level in seed coat besides at high level in fiber.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 03:19:01