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Titolo:
Expression of placental lactogen and cytokeratin in bovine placental binucleate cells in culture
Autore:
Nakano, H; Takahashi, T; Imai, K; Hashizume, K;
Indirizzi:
Natl Inst Anim Ind, Lab Reprod Endocrinol, Tsukuba, Ibaraki 3050901, JapanNatl Inst Anim Ind Tsukuba Ibaraki Japan 3050901 , Ibaraki 3050901, Japan
Titolo Testata:
CELL AND TISSUE RESEARCH
fascicolo: 2, volume: 303, anno: 2001,
pagine: 263 - 270
SICI:
0302-766X(200102)303:2<263:EOPLAC>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
PREGNANCY-ASSOCIATED GLYCOPROTEIN; SHEEP; LOCALIZATION; SERUM; RADIOIMMUNOASSAY; DIFFERENTIATION; IDENTIFICATION; PROGESTERONE; TROPHOBLAST; POPULATIONS;
Keywords:
binucleate cells; primary culture; placental lactogen; cytokeratin; placenta; cow;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Hashizume, K Natl Inst Anim Ind, Lab Reprod Endocrinol, Tsukuba Norin Kenkyu Danchi POB5, Tsukuba, Ibaraki 3050901, Japan Natl Inst Anim Ind Tsukuba Norin Kenkyu Danchi POB 5 Tsukuba Ibaraki Japan 3050901
Citazione:
H. Nakano et al., "Expression of placental lactogen and cytokeratin in bovine placental binucleate cells in culture", CELL TIS RE, 303(2), 2001, pp. 263-270

Abstract

Binucleate cells are present in ruminant placenta and play an endocrine role in the production of many hormones during pregnancy. We isolated and cultured binucleate cells from bovine placenta at middle to late gestation andcharacterized these cells using immunofluorescence techniques. Enriched preparations of binucleate cells were obtained using Percoll density gradientcentrifugation following collagenase digestion. Binucleate cells in culture preferentially attached to collagen-coated dishes rather than to noncoated plastic dishes. The cells gradually extended their edges on collagen substrata, and finally assumed a flattened morphology. Antibodies to placental lactogen (PL) and pregnancy-associated glycoprotein-1 (PAG-1) specifically stained the majority of round binucleate cells, but not the flat cells. We found that PL-positive binucleate cells were consistently devoid of cytokeratin. In contrast, cytokeratin was expressed in PL-negative binucleate cells as well as mononuclear epithelial cells. Furthermore, the PL-negative flat binucleate cells also developed intense cytokeratin networks in the cytoplasm. These results indicate that cytokeratin expression is inversely proportionate to that of PL in cultured binucleate cells. We conclude that downregulation of cytokeratin in binucleate cells is a function of the state of cellular differentiation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/08/20 alle ore 09:03:06