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Titolo:
Tissue microarray: A new technology for amplification of tissue resources
Autore:
Rimm, DL; Camp, RL; Charette, LA; Costa, J; Olsen, DA; Reiss, M;
Indirizzi:
Yale Univ, Sch Med, Dept Pathol, New Haven, CT 06510 USA Yale Univ New Haven CT USA 06510 ed, Dept Pathol, New Haven, CT 06510 USA Yale Univ, Sch Med, Dept Internal Med, New Haven, CT 06510 USA Yale Univ New Haven CT USA 06510 pt Internal Med, New Haven, CT 06510 USA Yale Univ, Sch Med, Yale Comprehens Canc Ctr, New Haven, CT 06510 USA YaleUniv New Haven CT USA 06510 rehens Canc Ctr, New Haven, CT 06510 USA
Titolo Testata:
CANCER JOURNAL
fascicolo: 1, volume: 7, anno: 2001,
pagine: 24 - 31
SICI:
1528-9117(200101/02)7:1<24:TMANTF>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
STORED PARAFFIN SLIDES; PROSTATE-CANCER; ESTROGEN-RECEPTOR; CARCINOMA;
Keywords:
immunoperoxidase; immunofluorescence; screening; histopathology; high-throughput validation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Rimm, DL Yale Univ, Sch Med, Dept Pathol, 310 Cedar St, New Haven, CT 06510 USA Yale Univ 310 Cedar St New Haven CT USA 06510 Haven, CT 06510 USA
Citazione:
D.L. Rimm et al., "Tissue microarray: A new technology for amplification of tissue resources", CANCER J, 7(1), 2001, pp. 24-31

Abstract

Tissue microarrays are a method of harvesting small disks of tissue from arange of standard histologic sections and placing them in an array on a recipient paraffin block such that hundreds of cases can be analyzed simultaneously, This technique allows maximization of tissue resources by analysis of small-core biopsies of blocks, rather than complete sections. Using thistechnology, a carefully planned array can be constructed with cases from pathology tissue block archives, such that a 20-year survival analysis can be performed on a cohort of 600 or more patients by use of only a few microliters of antibody in a single experiment. The reflex criticism of this technique is that the tissue analyzed is not representative, especially in antigens with heterogeneous staining patterns. This review addresses this issue, as well as the issue of antigen preservation or durability, which validates construction of arrays from archives. Strategies and methods of construction and analysis of the arrays are discussed, as well as some other unusual array applications. This technique can provide a highly efficient, high-throughput mechanism for evaluation of protein expression in large cohorts. It has the potential to allow validation of new genes at a speed comparableto the rapid rate of gene discovery afforded by DNA microarrays.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 17/01/21 alle ore 18:30:50