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Titolo:
Comparison of glucose and lactate as substrates during NMDA-induced activation of hippocampal slices
Autore:
Chih, CP; He, J; Sly, TS; Roberts, EL;
Indirizzi:
Miami VA Med Ctr, Geriatr Res Educ & Clin Ctr, Miami, FL 33125 USA Miami VA Med Ctr Miami FL USA 33125 Educ & Clin Ctr, Miami, FL 33125 USA Miami VA Med Ctr, Res Off, Miami, FL 33125 USA Miami VA Med Ctr Miami FL USA 33125 Med Ctr, Res Off, Miami, FL 33125 USA Univ Miami, Sch Med, Dept Neurol, Miami, FL 33136 USA Univ Miami Miami FLUSA 33136 , Sch Med, Dept Neurol, Miami, FL 33136 USA
Titolo Testata:
BRAIN RESEARCH
fascicolo: 1-2, volume: 893, anno: 2001,
pagine: 143 - 154
SICI:
0006-8993(20010302)893:1-2<143:COGALA>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAGNETIC-RESONANCE SPECTROSCOPY; VASCULAR SMOOTH-MUSCLE; RAT-BRAIN ASTROCYTES; HUMAN VISUAL-CORTEX; FREELY MOVING RAT; ENERGY-METABOLISM; NEURONAL-ACTIVITY; EXTRACELLULAR GLUCOSE; OXIDATIVE-METABOLISM; CULTURED ASTROCYTES;
Keywords:
alpha-cyano-4-hydroxycinnamate; intracellular pH; ion transport; N-methyl-D-aspartate; synaptic transmission; hippocampus; LDH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
82
Recensione:
Indirizzi per estratti:
Indirizzo: Roberts, EL Univ Miami, Sch Med, Dept Neurol, South Campus,Bldg B,Room 133C,12500 SW 152nd St, Miami, FL 33177 USA Univ Miami South Campus,Bldg B,Room 133C,12500 SW 152nd St Miami FL USA 33177
Citazione:
C.P. Chih et al., "Comparison of glucose and lactate as substrates during NMDA-induced activation of hippocampal slices", BRAIN RES, 893(1-2), 2001, pp. 143-154

Abstract

It has been postulated that lactate released from astrocytes may be the preferred metabolic substrate for neurons, particularly during intense neuronal activity (the astrocyte-neuron lactate shuttle hypothesis). We examined this hypothesis by exposing rat hippocampal slices to artificial cerebrospinal fluid containing either glucose or lactate and either N-methyl-D-aspartate, which activates neurons without stimulating astrocytic glucose uptake,or alpha -cyano-4-hydroxycinnamate, which blocks monocarboxylate transportacross plasma and mitochondrial membranes. When exposed to N-methyl-D-aspartate, slices lost synaptic transmission and K+ homeostasis more slowly in glucose-containing artificial cerebrospinal fluid than in lactate-containing artificial cerebrospinal fluid. After N-methyl-D-aspartate exposure, slices recovered synaptic transmission more completely in glucose. These results suggest that hippocampal neurons can use glucose more effectively than lactate when energy demand is high. In experiments with alpha -cyano-4-hydroxycinnamate, 500 muM alpha -cyano-4-hydroxycinnamate caused loss of K+ homeostasis and synaptic transmission in hippocampal slices during normoxia. When 200 muM alpha -cyano-4-hydroxycinnamate was used, synaptic activity and intracellular pH in slices decreased significantly during normoxia. These results suggest that alpha -cyano-4-hydroxycinnamate may have blocked mitochondrial oxidative metabolism along with lactate transport. Thus, studies using alpha -cyano-4-hydroxycinnamate to demonstrate the presence of a lactateshuttle in the brain tissue may need reevaluation. Our findings, together with observations in the literature that (1) glucose is available to neurons during activation, (2) heightened energy demand rapidly activates glycolysis in neurons, and (3) activation of glycolysis suppresses lactate utilization, suggests that glucose is the primary substrate for neurons during neuronal activation and do not support the astrocyte-neuron lactate shuttle hypothesis. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 09:28:23