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Titolo:
Coherent scattering in multi-harmonic light microscopy
Autore:
Moreaux, L; Sandre, O; Charpak, S; Blanchard-Desce, M; Mertz, J;
Indirizzi:
ESPCI, INSERM EPI 00 02, Neurophysiol Lab, F-75005 Paris, France ESPCI Paris France F-75005 0 02, Neurophysiol Lab, F-75005 Paris, France Inst Curie, Lab Physicochim, CNRS, UMR 168, Paris, France Inst Curie Paris France , Lab Physicochim, CNRS, UMR 168, Paris, France Univ Rennes, CNRS UMR 6510, Lab Synthese & Electrosynthese Organ, Rennes, France Univ Rennes Rennes France these & Electrosynthese Organ, Rennes, France
Titolo Testata:
BIOPHYSICAL JOURNAL
fascicolo: 3, volume: 80, anno: 2001,
pagine: 1568 - 1574
SICI:
0006-3495(200103)80:3<1568:CSIMLM>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHARGE-SHIFT PROBES; 2ND-HARMONIC GENERATION; 2-PHOTON ABSORPTION; FLUORESCENCE MICROSCOPY; ORGANIC-MOLECULES; CROSS-SECTIONS; MEMBRANE; EXCITATION; HYPERPOLARIZABILITY; CULTURES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Mertz, J ESPCI, INSERM EPI 00 02, Neurophysiol Lab, 10 Rue Vauquelin, F-75005 Paris, France ESPCI 10 Rue Vauquelin Paris France F-75005 -75005 Paris, France
Citazione:
L. Moreaux et al., "Coherent scattering in multi-harmonic light microscopy", BIOPHYS J, 80(3), 2001, pp. 1568-1574

Abstract

By focusing a pulsed laser beam into a sample, harmonic up-conversion can be generated as well as multi-photon excited fluorescence. Whereas multi-photon excited fluorescence microscopy is well established, the use of multi-harmonic generation for three-dimensional image contrast is very recent. Both techniques can provide similar resolution and, for adequate radiating source density, comparable signal levels, allowing them to be combined in a single versatile instrument.; However, harmonic generation differs fundamentally from fluorescence generation in that it is coherent and produces radiation patterns that are highly sensitive to phase. As such, multi-harmonic generation microscopy provides a unique window into molecular spatial organization that is inaccessible to fluorescence.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 09:18:17