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Titolo:
Quantitative measurements of mixing intensity in shake-flasks and stirred tank reactors - Use of the Mixmeter, a mixing process analyzer
Autore:
Gerson, DF; Kole, MM;
Indirizzi:
Wyeth Lederle Vaccines, Pearl River, NY 10965 USA Wyeth Lederle Vaccines Pearl River NY USA 10965 Pearl River, NY 10965 USA
Titolo Testata:
BIOCHEMICAL ENGINEERING JOURNAL
fascicolo: 2, volume: 7, anno: 2001,
pagine: 153 - 156
SICI:
1369-703X(200103)7:2<153:QMOMII>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Keywords:
mixing signal; root mean square; reactor system; mixing; shake-flask;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
4
Recensione:
Indirizzi per estratti:
Indirizzo: Gerson, DF Wyeth Lederle Vaccines, 401 N Middletown Rd, Pearl River, NY 10965 USA Wyeth Lederle Vaccines 401 N Middletown Rd Pearl River NY USA 10965
Citazione:
D.F. Gerson e M.M. Kole, "Quantitative measurements of mixing intensity in shake-flasks and stirred tank reactors - Use of the Mixmeter, a mixing process analyzer", BIOCH ENG J, 7(2), 2001, pp. 153-156

Abstract

A new mixing probe has been developed which measures the motions of the fluid during mixing as pressure fluctuations and converts the measurements into a mixing signal (MS). The MS is the root mean square (RMS) pressure fluctuation in the 1-64 Hz range as determined by a sensitive pressure sensor and a digital signal processor specifically designed for the purpose. The MSis a measure of the actual mixing flow of the fluid rather than a measurement of the input motions or energies into the reactor system (e.g. RPM, torque or power). In other studies, the MS has been measured as a function of mixing speed in numerous sized reactors from 10 to 1000 I, and provides consistent and reproducible measurements. The MS increases monotonically as a function of mixing speed, with a change of slope corresponding to the transition from laminar to turbulent mixing regimes. Maps of MS as a function oflocation in the reactor are useful in understanding stirred tank reactor design and performance. Quantitative measurements of mixing are especially useful during process development as a tool to increase the success of scale-up during the transition from process development to manufacturing. Measurements at a fixed location in a given reactor are useful in understanding changes in mixing that occur during the course of a given process, and are useful in manufacturing situations where validated documentation of lot-to-lot consistency of mixing is required (e.g. pharmaceutical manufacturing). Tn addition, the probe has been used to measure mixing in vessels with vibrational mixers with similar results, The probe has been successfully used infeedback loops to control either mixing speed or vibrational mixing amplitude in order to maintain constant mixing of the fluid during processing. With this system it is possible to maintain constant mixing over a wide rangeof fluid volumes in a given reactor, and, for instance, to compensate for changes in viscosity throughout the course of the process. Adaptations of this system for the measurement of mixing in shake-flasks is described in this paper. (C) 2001 Published by Elsevier Science B.V.

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Documento generato il 02/07/20 alle ore 18:20:31