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Titolo:
Identification of leukemia inhibitory factor as a potent mast cell growth-enhancing factor produced by mouse keratinocyte cell line, KCMH-1
Autore:
Tanaka, T; Morita, E; Mihara, S; Kanno, M; Yamamoto, S;
Indirizzi:
Hiroshima Univ, Fac Med, Dept Dermatol, Minami Ku, Hiroshima 7348551, Japan Hiroshima Univ Hiroshima Japan 7348551 nami Ku, Hiroshima 7348551, Japan Hiroshima Univ, Fac Med, Dept Immunol & Parasitol, Minami Ku, Hiroshima 7348551, Japan Hiroshima Univ Hiroshima Japan 7348551 nami Ku, Hiroshima 7348551, Japan
Titolo Testata:
ARCHIVES OF DERMATOLOGICAL RESEARCH
fascicolo: 1-2, volume: 293, anno: 2001,
pagine: 18 - 25
SICI:
0340-3696(200102)293:1-2<18:IOLIFA>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
BONE-MARROW CELLS; EMBRYONIC STEM-CELLS; CONDITIONED MEDIUM; DIFFERENTIATION; PURIFICATION; CARCINOMA; MICE; INDUCTION; DISTINCT; CYTOKINE;
Keywords:
mast cell; leukemia inhibitory factor; keratinocyte; fibroblast; KCMH-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Tanaka, T Hiroshima Univ, Fac Med, Dept Dermatol, Minami Ku, 1-2-3 Kasumi,Hiroshima7348551, Japan Hiroshima Univ 1-2-3 Kasumi Hiroshima Japan 7348551 8551, Japan
Citazione:
T. Tanaka et al., "Identification of leukemia inhibitory factor as a potent mast cell growth-enhancing factor produced by mouse keratinocyte cell line, KCMH-1", ARCH DERM R, 293(1-2), 2001, pp. 18-25

Abstract

Inoculation of KCMH-1 cells, a keratinocyte-derived cell line established from a chemically induced skin tumor, into the skin of mice results in accumulation of mast cells around the resulting tumors, The conditioned medium of KCMH-1 cells enhances the growth of mast cells in vitro when they are cultured in the presence of NIH/3T3 fibroblasts, suggesting an important rolefor keratinocytes in mast cell hyperplasia in the skin, The aim of this study was to identify this mast cell growth-enhancing factor (MCGEF) by screening a KCMH-1 cDNA library. We first established a polyclonal antibody raised against the partially purified factor obtained from KCMH-1-conditioned medium which neutralized the MCGEF activity in KCMH-1-conditioned medium. Expression cloning of 1 X 10(6) cDNAs from the KCMH-1 cDNA library led to 16 cDNAs, One of these cloned cDNAs was found to be leukemia inhibitory factor(LIF). Both LIF produced by COS cells and the recombinant protein obtainedcommercially showed MCGEF activity when added to mast cell/fibroblast cocultures, MCGEF activity in KCMH-1-conditioned medium was completely neutralized by an anti-LIE monoclonal antibody, These results suggest that MCGEF produced by KCMH-1 cells is identical to LIF.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 20:08:02