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Titolo:
Anaerobic degradation of 2-methylnaphthalene by a sulfate-reducing enrichment culture
Autore:
Annweiler, E; Materna, A; Safinowski, M; Kappler, A; Richnow, HH; Michaelis, W; Meckenstock, RU;
Indirizzi:
Univ Konstanz, Fak Biol, D-78457 Constance, Germany Univ Konstanz Constance Germany D-78457 Biol, D-78457 Constance, Germany Univ Hamburg, Inst Biogeochem & Meereschem, D-20146 Hamburg, Germany Univ Hamburg Hamburg Germany D-20146 eereschem, D-20146 Hamburg, Germany UFZ, Umweltforschungszentrum Leipzig Halle GMBH, D-04318 Leipzig, Germany UFZ Leipzig Germany D-04318 Leipzig Halle GMBH, D-04318 Leipzig, Germany
Titolo Testata:
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
fascicolo: 12, volume: 66, anno: 2000,
pagine: 5329 - 5333
SICI:
0099-2240(200012)66:12<5329:ADO2BA>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYCYCLIC AROMATIC-HYDROCARBONS; DENITRIFYING BACTERIUM; NAPHTHALENE DEGRADATION; ENZYMATIC REDUCTION; THAUERA-AROMATICA; ANOXIC CONDITIONS; KEY REACTION; BENZOYL-COA; STRAIN-T; TOLUENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Meckenstock, RU Univ Konstanz, Fak Biol, Univ Str 10, D-78457 Constance, Germany Univ Konstanz Univ Str 10 Constance Germany D-78457 rmany
Citazione:
E. Annweiler et al., "Anaerobic degradation of 2-methylnaphthalene by a sulfate-reducing enrichment culture", APPL ENVIR, 66(12), 2000, pp. 5329-5333

Abstract

Anaerobic degradation of 2-methylnaphthalene was investigated with a sulfate-reducing enrichment culture. Metabolite analyses revealed two groups of degradation products. The first group comprised two succinic acid adducts which were identified as naphthyl-2-methyl-succinic acid and naphthyl-2-methylene-succinic acid by comparison with chemically synthesized reference compounds. Naphthyl-2-methyl-succinic acid accumulated to 0.5 muM in culture supernatants. Production of naphthyl-2-methyl-succinic acid was analyzed in enzyme assays with dense cell suspensions. The conversion of 2-methylnaphthalene to naphthyl-2-methyl-succinic acid was detected at a specific activity of 0.020 +/- 0.003 nmol min(-1) mg of protein(-1) only in the presence ofcells and fumarate. We conclude that under anaerobic conditions 2-methylnaphthalene is activated by fumarate addition to the methyl group, as is the case in anaerobic toluene degradation. The second group of metabolites comprised 2-naphthoic acid and reduced 2-naphthoic acid derivatives, including 5,6,7,8-tetrahydro-2-naphthoic acid, octahydro-2-naphthoic acid, and decahydro-2-naphthoic acid. These compounds were also identified in an earlier study as products of anaerobic naphthalene degradation with the same enrichment culture. A pathway for anaerobic degradation of 2-methylnaphthalene analogous to that for anaerobic toluene degradation is proposed.

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Documento generato il 15/07/20 alle ore 08:00:31