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Titolo:
Metallo-beta-lactamase producers in environmental microbiota: New molecular class B enzyme in Janthinobacterium lividum
Autore:
Rossolini, GM; Condemi, MA; Pantanella, F; Docquier, JD; Amicosante, G; Thaller, MC;
Indirizzi:
Univ Siena, Dipartimento Biol Mol, Sez Microbiol, I-53100 Siena, Italy Univ Siena Siena Italy I-53100 Mol, Sez Microbiol, I-53100 Siena, Italy Univ La Sapienza, Ist Microbiol, I-00185 Rome, Italy Univ La Sapienza Rome Italy I-00185 , Ist Microbiol, I-00185 Rome, Italy Univ Aquila, Dipartimento Sci & Tecnol Biomed, I-67100 Laquila, Italy UnivAquila Laquila Italy I-67100 Tecnol Biomed, I-67100 Laquila, Italy Univ Roma Tor Vergata 2, Dipartimento Biol, I-00133 Rome, Italy Univ Roma Tor Vergata 2 Rome Italy I-00133 nto Biol, I-00133 Rome, Italy
Titolo Testata:
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
fascicolo: 3, volume: 45, anno: 2001,
pagine: 837 - 844
SICI:
0066-4804(200103)45:3<837:MPIEMN>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
AERUGINOSA CLINICAL ISOLATE; PSEUDOMONAS-AERUGINOSA; BACILLUS-CEREUS; BACTEROIDES-FRAGILIS; CRYSTAL-STRUCTURE; ESCHERICHIA-COLI; GENE BLA(IMP); RESISTANCE; CARBAPENEMASE; CLONING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Rossolini, GM Univ Siena, Dipartimento Biol Mol, Sez Microbiol, Via Laterina 8, I-53100 Siena, Italy Univ Siena Via Laterina 8 Siena Italy I-53100 Siena, Italy
Citazione:
G.M. Rossolini et al., "Metallo-beta-lactamase producers in environmental microbiota: New molecular class B enzyme in Janthinobacterium lividum", ANTIM AG CH, 45(3), 2001, pp. 837-844

Abstract

Eleven environmental samples from different sources were screened for the presence of metallo-beta -lactamase-producing bacteria by using a selectiveenrichment medium containing a carbapenem antibiotic and subsequently testing each isolate for production of EDTA-inhibitable carbapenemase activity. A total of 15 metallo-beta -lactamase-producing isolates, including 10 Stenotrophomonas maltophilia isolates, 3 Chlyseobacterium spp,, one Aeromonas hydrophila isolate, and one Janthinobacterium lividum isolate (a species inwhich production of metallo-beta -lactamase activity was not previously reported), were obtained from 8 samples. In the J, lividum isolate, named JAC1, production of metallo-beta -lactamase activity was elicited upon exposure to beta -lactams, Screening of a JAC1 genomic library for clones showing a reduced imipenem susceptibility led to the isolation of a metallo-beta -lactamase determinant encoding a new member (named THIN-B) of the highly divergent subclass B3 lineage of metallo-beta -lactamases. THIN-B is most closely related (35.6% identical residues) to the L1 enzyme of S, maltophilia and more distantly related to the FEZ-1 enzyme of Legionella gormanii (27.8%identity) and to the GOB-1 enzyme of Chryseobacterium meningosepticum (24.2% identity). Sequences related to bla(THIN-B), and inducible production ofmetallo-beta -lactamase activity, were also detected in the J. lividum type strain DSM1522. Expression of the bla(THIN-B), gene in Escherichia coli resulted in decreased susceptibility to several beta -lactams, including penicillins, cephalosporins (including cephamycins and oxyimino cephalosporins), and carbapenems, revealing a broad substrate specificity of the enzyme. The results of this study indicated that metallo-beta -lactamase-producing bacteria are widespread in the environment and identified a new molecular class B enzyme in the environmental species J, lividum.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 08:41:17