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Titolo:
Cryopreservation of shoot tips from six endangered Australian species using a modified vitrification protocol
Autore:
Turner, SR; Senaratna, T; Bunn, E; Tan, B; Dixon, KW; Touchell, DH;
Indirizzi:
Kings Pk & Bot Garden, W Perth, WA 6005, Australia Kings Pk & Bot Garden W Perth WA Australia 6005 Perth, WA 6005, Australia Curtin Univ Technol, Bentley, WA 6102, Australia Curtin Univ Technol Bentley WA Australia 6102 Bentley, WA 6102, Australia ARS, USDA, Natl Seed Storage Lab, Ft Collins, CO 80521 USA ARS Ft CollinsCO USA 80521 tl Seed Storage Lab, Ft Collins, CO 80521 USA
Titolo Testata:
ANNALS OF BOTANY
fascicolo: 3, volume: 87, anno: 2001,
pagine: 371 - 378
SICI:
0305-7364(200103)87:3<371:COSTFS>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
WASABI WASABIA-JAPONICA; PLANT-REGENERATION; CELLS; MERISTEMS; SURVIVAL; L.;
Keywords:
A. humilis ssp chrysanthus; A. kalbarriensis; A. viridis ssp terraspectans; Conostylis dielsia ssp teres; C. micrantha; C. wonganensis; kangaroo paws; Haemodoraceae; vitrification; cryopreservation; rare and endangered; conservation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Turner, SR Kings Pk & Bot Garden, W Perth, WA 6005, Australia Kings Pk & Bot Garden W Perth WA Australia 6005 005, Australia
Citazione:
S.R. Turner et al., "Cryopreservation of shoot tips from six endangered Australian species using a modified vitrification protocol", ANN BOTANY, 87(3), 2001, pp. 371-378

Abstract

An efficient vitrification procedure was developed and successfully applied to cryopreserve six endangered West Australian species(family Haemodoraceae: Anigozanthos humilis ssp. chrysanthus Hopper: A. kalbarriensis Hopper: A. viridis ssp. terraspectans Hopper: Conostylis dielsia ssp. teres Hopper:C. micrantha Hopper and C. wonganensis Hopper). Species were initially evaluated for cryostorage using a basic vitrification protocol involving: culturing plantlets in vitro for 21 d; excision of shoot apices: preculture of apical tips on 0.4 M sorbitol for 2 d. followed by incubation in PVS2 (plant vitrification solution 2) for 25 min at 0 degreesC, then direct immersionin liquid nitrogen (LN). Warming of retrieved material was for 1 min in a 40 degreesC water bath. Using this protocol five of the six species exhibited low post-storage survival, while the sixth species, A. viridis ssp. terraspectans posted higher survival (61.1%). Using A. viridis ssp. terraspectans as an indicator species, the initial protocol was modified to include: 3d preculture on 0.80 M glycerol, loading treatment with 2 0 M glycerol plus 0.4 M sucrose solution for 20 min, followed by 25 min exposure to a modified PVS2. Survival was significantly improved in the test species, and in further experiments three other species also showed significant improvementswith the new protocol. Key findings include: effectiveness of glycerol in the preculture medium; the effect of preculture duration; the importance ofa loading stage for these species: and the successful use of modified PVS2solutions with reduced or zero dimethyl sulfoxide (DMSO). (C) 2001 Annals of Botany Company.

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Documento generato il 16/07/20 alle ore 05:48:41