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Titolo:
Changes in androgenic steroid profile due to urine contamination by microorganisms: A prospective study in the context of doping control
Autore:
de la Torre, R; de la Torre, X; Alia, C; Segura, J; Baro, T; Torres-Rodriguez, JM;
Indirizzi:
Univ Autonoma Barcelona, Inst Municipal Invest Med, Pharmacol Res Unit, E-08003 Barcelona, Spain Univ Autonoma Barcelona Barcelona Spain E-08003 E-08003 Barcelona, Spain Univ Autonoma Barcelona, Inst Municipal Invest Med, Microbiol Res Unit, E-08003 Barcelona, Spain Univ Autonoma Barcelona Barcelona Spain E-08003 E-08003 Barcelona, Spain Univ Pompeu Fabra, Barcelona, Spain Univ Pompeu Fabra Barcelona SpainUniv Pompeu Fabra, Barcelona, Spain
Titolo Testata:
ANALYTICAL BIOCHEMISTRY
fascicolo: 2, volume: 289, anno: 2001,
pagine: 116 - 123
SICI:
0003-2697(20010215)289:2<116:CIASPD>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
MASS-SPECTROMETRIC ANALYSIS; TESTOSTERONE; DIHYDROTESTOSTERONE; EPITESTOSTERONE; EXPERIENCE;
Keywords:
urinary; contamination; testosterone; 4-androstene-3,17-dione; 5 alpha-androstane-3,17-dione; 5 beta-androstane-3,17-dione; epitestosterone; doping in sports; androgenic steroid profile; results interpretation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: de la Torre, R Univ Autonoma Barcelona, Inst Municipal Invest Med, Pharmacol Res Unit, Doctor Aiguader 80, E-08003 Barcelona, Spain Univ Autonoma Barcelona Doctor Aiguader 80 Barcelona Spain E-08003
Citazione:
R. de la Torre et al., "Changes in androgenic steroid profile due to urine contamination by microorganisms: A prospective study in the context of doping control", ANALYT BIOC, 289(2), 2001, pp. 116-123

Abstract

Urine contamination by microorganisms may affect the interpretation of urinalysis in different areas of clinical diagnosis. This is particularly relevant in doping control. A prospective study was designed to assess the effects of urine contamination by selected pathogens on the endogenous androgenic steroid profile. Pooled urine from a healthy male volunteer with standard steroid profile compared with reference values for the Caucasian population was sterilized by filtration and stored in sterile glass tubes. Aliquotswere inoculated with known amounts of 15 different organisms (bacteria, fungi, and moulds) and incubated at 37 degreesC for 2 weeks. Different markers of urine contamination, such as pH, deconjugation of steroids, and metabolic by-products, were determined. Alkalization of urinary pH was not a reliable indicator of urine contamination as several organisms grew in this medium and no alteration of this parameter was found. In uncontaminated urine,less than 10% of steroid glucuronide conjugates were spontaneously hydrolyzed. Higher rates of hydrolysis for sulfate conjugates were found. An unconjugated fraction higher than 10% of the total amount of testosterone was a reliable indicator of urine contamination. However, microbial production oftestosterone or epitestosterone was not detected. In contrast, a few organisms were able to synthesize 5 alpha -androstanedione, 5 beta -androstanedione, and androstenedione using endogenous steroids as substrates, (C) 2001 Academic Press.

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Documento generato il 03/06/20 alle ore 02:39:57