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Titolo:
Crystallization and preliminary X-ray analysis of a novel pectolytic enzyme, polymethoxygalacturonase SX1 from Trichosporon penicillatum
Autore:
Tada, T; Lu, CT; Nakamura, Y; Wada, K; Miyahara, I; Hirotsu, K; Katsuya, Y; Sawada, M; Takao, M; Sakai, T; Nishimura, K;
Indirizzi:
Univ Osaka Prefecture, Res Inst Adv Sci & Technol, Osaka 5998570, Japan Univ Osaka Prefecture Osaka Japan 5998570 Technol, Osaka 5998570, Japan Osaka City Univ, Dept Chem, Sumiyoshi Ku, Osaka 5588585, Japan Osaka City Univ Osaka Japan 5588585 , Sumiyoshi Ku, Osaka 5588585, Japan Hyogo Prefectural Inst Ind Res, Suma Ku, Kobe, Hyogo 6540037, Japan Hyogo Prefectural Inst Ind Res Kobe Hyogo Japan 6540037 go 6540037, Japan Godo Shusei Co Ltd, Cent Res Lab, Chiba 2710064, Japan Godo Shusei Co LtdChiba Japan 2710064 ent Res Lab, Chiba 2710064, Japan Kinki Univ, Dept Food Sci, Nara 6318505, Japan Kinki Univ Nara Japan 6318505 i Univ, Dept Food Sci, Nara 6318505, Japan
Titolo Testata:
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
, volume: 57, anno: 2001,
parte:, 3
pagine: 457 - 458
SICI:
0907-4449(200103)57:<457:CAPXAO>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
PECTATE LYASE; 3-DIMENSIONAL STRUCTURE; ASPERGILLUS-NIGER; CRYSTAL-STRUCTURE; POLYGALACTURONASE; PECTIN; RESOLUTION; PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Tada, T Univ Osaka Prefecture, Res Inst Adv Sci & Technol, Osaka 5998570, Japan Univ Osaka Prefecture Osaka Japan 5998570 , Osaka 5998570, Japan
Citazione:
T. Tada et al., "Crystallization and preliminary X-ray analysis of a novel pectolytic enzyme, polymethoxygalacturonase SX1 from Trichosporon penicillatum", ACT CRYST D, 57, 2001, pp. 457-458

Abstract

A novel pectolytic enzyme, polymethoxygalacturonase SX1 from Trichosporon penicillatum, with a molcular weight of 36 kDa was crystallized by the hanging-drop vapour-diffusion method using polyethylene glycol 1000 as a precipitant. The crystals belonged to the monoclinic space group C2, with unit-cell parameters a = 165.6, b = 61.0, c = 48.7 Angstrom, beta = 93.1 degrees. The calculated V-M based on one molecule per asymmetric unit was 3.40 Angstrom (3) Da(-1). A native data set was collected to 2.08 Angstrom resolutionfrom a crystal on a Cu K alpha rotating-anode X-ray source. A molecular-replacement solution was obtained using the program AMoRe and the structure of endopolygalacturonase II from Aspergillus niger as a model.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 17:29:31