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Titolo:
Oral transgene vaccination mediated by attenuated Salmonellae is an effective method to prevent Herpes simplex virus-2a induced disease in mice
Autore:
Flo, J; Tisminetzky, S; Baralle, F;
Indirizzi:
Int Ctr Genet Engn & Biotechnol, Area Sci Pk, I-34012 Trieste, Italy Int Ctr Genet Engn & Biotechnol Trieste Italy I-34012 012 Trieste, Italy
Titolo Testata:
VACCINE
fascicolo: 13-14, volume: 19, anno: 2001,
pagine: 1772 - 1782
SICI:
0264-410X(20010208)19:13-14<1772:OTVMBA>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
MUCOSAL IMMUNITY; GLYCOPROTEIN-D; PROTECTIVE IMMUNITY; DNA IMMUNIZATION; VAGINAL INFECTION; TYPE-2 INFECTION; GENITAL HERPES; IFN-GAMMA; INTERFERON; TYPHIMURIUM;
Keywords:
vaccination; mice; Salmonellae; HSV; DNA vaccine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Baralle, F Int Ctr Genet Engn & Biotechnol, Area Sci Pk, Padriciano 99, I-34012 Trieste, Italy Int Ctr Genet Engn & Biotechnol Padriciano 99 TriesteItaly I-34012
Citazione:
J. Flo et al., "Oral transgene vaccination mediated by attenuated Salmonellae is an effective method to prevent Herpes simplex virus-2a induced disease in mice", VACCINE, 19(13-14), 2001, pp. 1772-1782

Abstract

An attenuated strain of Salmonella typhimurium has been used as a carrier for oral genetic immunization. The eukaryotic expression vector pCMV containing the gene of the glycoprotein D (gD) Of the herpes simplex virus 2 was used to transform Salmonellae. The oral immunization with the transformed salmonellae elicited a strong cellular immune response in both, the mucosal and systemic compartments (spleen, ileal lymph nodes and Peyer patches). The immune response mainly consisted in a dramatic activation of IFN-gamma -secreting cells. Twenty hours following the challenge with five lethal dosesof virus, mRNA for IFN-gamma was observed in vaginal tissues from mice immunized with salmonella harboring the plasmid pgD but not in tissues from mice immunized by the intramuscular route with pgD. After an intravaginal challenge all immunized mice survived without developing symptoms. Furthermore, the immunization with Salmonella resulted in a more effective control of viral shedding than intramuscular immunization. We have unequivocally demonstrated by the introduction of an intron in the green fluorescent protein that the expression of the plasmid was due to the transcription of the protein by an eukaryotic nuclear process and not as a result of expression of the protein by the bacteria. Macrophages and dendritic cells were found expressing the protein in systemic and mucosal compartments of the immune system. (C) 2001 Elsevier Science Ltd. All rights reserved.

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Documento generato il 15/07/20 alle ore 21:19:18