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Titolo:
Whole blood tissue factor procoagulant activity remains detectable during severe aplasia following bone marrow and peripheral blood stem cell transplantation
Autore:
Ozcan, M; Morton, CT; Solovey, A; Dandelet, L; Bach, RR; Hebbel, RP; Slungaard, A; Key, NS;
Indirizzi:
Univ Minnesota, Sch Med, Dept Med, Minneapolis, MN 55455 USA Univ Minnesota Minneapolis MN USA 55455 pt Med, Minneapolis, MN 55455 USA Ankara Univ, Sch Med, Dept Hematol, Ibni Sina Hosp, TR-06100 Ankara, Turkey Ankara Univ Ankara Turkey TR-06100 ni Sina Hosp, TR-06100 Ankara, Turkey Vet Adm Hosp, Res Serv, Minneapolis, MN USA Vet Adm Hosp Minneapolis MN USA Adm Hosp, Res Serv, Minneapolis, MN USA
Titolo Testata:
THROMBOSIS AND HAEMOSTASIS
fascicolo: 2, volume: 85, anno: 2001,
pagine: 250 - 255
SICI:
0340-6245(200102)85:2<250:WBTFPA>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR NECROSIS FACTOR; ENDOTHELIAL-CELLS; FACTOR EXPRESSION; COAGULANT ACTIVITY; GAMMA-INTERFERON; GROWTH-FACTOR; IN-SITU; MONOCYTES; ENDOTOXIN; DISEASE;
Keywords:
tissue factor; whole blood; bone marrow transplantation; bone marrow aplasia;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Key, NS Univ Minnesota, Sch Med, Dept Med, Box 480 Mayo,420 Delaware St SE, Minneapolis, MN 55455 USA Univ Minnesota Box 480 Mayo,420 Delaware St SE Minneapolis MN USA 55455
Citazione:
M. Ozcan et al., "Whole blood tissue factor procoagulant activity remains detectable during severe aplasia following bone marrow and peripheral blood stem cell transplantation", THROMB HAEM, 85(2), 2001, pp. 250-255

Abstract

Using a novel whole blood assay, wt: recently demonstrated that tissue factor procoagulant activity (TF PCA) is present in normal individuals. Preliminary experiments suggested that this activity is localized in the mononuclear cell fraction. Postulating that whole blood TF PCA would therefore be undetectable when monocytes and neutrophils are absent from peripheral blood, we assayed TF PCA during the peri-transplant period in 15 consecutive patients undergoing allogeneic (n = 12) or autologous (n = 3) bone marrow transplantation (BMT) or peripheral blood stem cell transplantation (PBSCT). Baseline (pre-transplant) mean TF PCA was higher in patients compared to normal controls (P <0.005). Unexpectedly, although TF PCA during the period of profound aplasia was significantly reduced compared to baseline (p <0.05), fully 55% of the initial activity remained detectable. During the engraftment phase, TF PCA returned to pre-transplant levels, with a linear correlation between monocyte counts and TF PCA (r = 0.63). In contrast to normal whole blood, incubation of aplastic samples with E. Coil lipopolysaccharide exvivo failed to induce TF PCA. Throughout the period of study - but especially during the aplastic phase - the absolute number of circulating endothelial cells (CECs) that were TF antigen-positive was increased compared to normals (P <0.001). However, removal of these cells from whole blood samples failed to significantly diminish total TF PCA indicating that CECs alone could not account for the detectable TF PCA during aplasia. We conclude that neither circulating mature myelo-monocytic cells nor endothelial cells can account for all the functionally intact TF in peripheral blood. Further studies are needed to identify the other source(s) of TF PCA.

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Documento generato il 31/03/20 alle ore 10:23:48