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Titolo:
Identification and characterization of B-cell epitopes of a 53-kDa outer membrane protein from Porphyromonas gingivalis
Autore:
Oyaizu, K; Ohyama, H; Nishimura, F; Kurihara, H; Matsushita, S; Maeda, H; Kokeguchi, S; Hongyo, H; Takashiba, S; Murayama, Y;
Indirizzi:
Okayama Univ, Sch Dent, Dept Periodontol & Endocrinol, Okayama 7008525, Japan Okayama Univ Okayama Japan 7008525 & Endocrinol, Okayama 7008525, Japan Kumamoto Univ, Grad Sch Med Sci, Dept Neurosci & Immunol, Div Immunogenet,Kumamoto 860, Japan Kumamoto Univ Kumamoto Japan 860 ol, Div Immunogenet,Kumamoto 860, Japan
Titolo Testata:
ORAL MICROBIOLOGY AND IMMUNOLOGY
fascicolo: 2, volume: 16, anno: 2001,
pagine: 73 - 78
SICI:
0902-0055(200104)16:2<73:IACOBE>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAPIDLY PROGRESSIVE PERIODONTITIS; EARLY-ONSET PERIODONTITIS; BACTEROIDES-GINGIVALIS; SERUM ANTIBODIES; T-CELL; DISEASE; IGG; RESPONSES;
Keywords:
Porphyromonas gingivalis; outer membrane protein; B-cell epitopes; serology; peptides;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Murayama, Y Okayama Univ, Sch Dent, Dept Periodontol & Endocrinol, 2-5-1 Shikata Cho, Okayama 7008525, Japan Okayama Univ 2-5-1 Shikata Cho Okayama Japan 7008525 5, Japan
Citazione:
K. Oyaizu et al., "Identification and characterization of B-cell epitopes of a 53-kDa outer membrane protein from Porphyromonas gingivalis", ORAL MICROB, 16(2), 2001, pp. 73-78

Abstract

We have previously reported that Porphyromonas gingivalis FDC 381 possesses a 53-kDa protein antigen (Ag53) on its outer membrane that evokes a strong humoral immune response in many patients with periodontal disease and that the humoral immune responses to Ag53 differ greatly among patients. To understand how the individual humoral immune system against Ag53 was determined, the regions of Ag53 recognized by specific antibody (B-cell epitopes) and dominant subclasses of serum immunoglobulin G (IgG) against major B-cellepitopes were examined by enzyme-linked immunosorbent assay. This study used sera from six patients with periodontitis, which all reacted strongly with sonic extracts of P. gingivalis 381 and with purified Ag53, and sera from six periodontally healthy children, which did not react with either sonicextracts of P. gingivalis 381 or Ag53. The epitopes were identified using synthetic 5-residue overlapping decapeptides covering the entire Ag53. Thirteen of 89 synthetic decapeptides showed a strong reaction with sera from the periodontal patients, but no reaction with those from the healthy children. Four peptides of 13 exerted different immune responses among patients. Furthermore, restriction analyses of the highly antigenic regions revealed that three sequences, RAAIRAS, YYLQ and MSPARR, were identified as major B-cell epitopes. Additionally these epitopes were recognized mainly by the IgG2 isotype. These data suggest that the difference of B-cell epitopes mightinfluence individual differences in antibody titer against Ag53 and also that the epitopes recognized commonly by multiple antibodies are quite valuable for peptide vaccine development against P. gingivalis infection.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 13:37:48