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Titolo:
Immobilization of oligodeoxyribonucleotides with multiple anchors to microchips
Autore:
Zhao, XD; Nampalli, S; Serino, AJ; Kumar, S;
Indirizzi:
Amersham Pharmacia Biotech Inc, Piscataway, NJ 08855 USA Amersham Pharmacia Biotech Inc Piscataway NJ USA 08855 away, NJ 08855 USA
Titolo Testata:
NUCLEIC ACIDS RESEARCH
fascicolo: 4, volume: 29, anno: 2001,
pagine: 955 - 959
SICI:
0305-1048(20010215)29:4<955:IOOWMA>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
OLIGONUCLEOTIDE ARRAYS; DNA-SYNTHESIS; HYBRIDIZATION; TEMPLATE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Kumar, S Amersham Pharmacia Biotech Inc, 800 Centennial Ave, Piscataway, NJ 08855 USA Amersham Pharmacia Biotech Inc 800 Centennial Ave Piscataway NJUSA 08855
Citazione:
X.D. Zhao et al., "Immobilization of oligodeoxyribonucleotides with multiple anchors to microchips", NUCL ACID R, 29(4), 2001, pp. 955-959

Abstract

Facile modification of oligodeoxyribonucleotides is required for efficientimmobilization to a pre-activated glass surface, This report presents an oligodeoxyribonucleotide which contains a hairpin stem-loop structure with multiple phosphorothioate moieties in the loop. These moieties are used to anchor the oligo to glass slides that are pre-activated with bromo-acetamidopropylsilane. The efficiency of the attachment reaction was improved by increasing the number of phosphorothioates in the loop, as shown in the remarkable enhancement of template hybridization and single base extension through catalysis by DNA polymerase. The loop and stem presumably serve as lateral spacers between neighboring oligodeoxyribonucleotides and as a linker armbetween the glass surface and the single-stranded sequence of interest. The oligodeoxyribonucleotides of this hairpin stem-loop architecture with multiple phosphorothioate moieties have broad application in DNA chip-based gene analysis.

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Documento generato il 26/01/20 alle ore 22:18:17