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Titolo:
Chronic morphine treatment inhibits oxytocin release from the supraoptic nucleus slices of rats
Autore:
Li, JH; You, ZD; Chen, ZY; Song, CY; Lu, CL;
Indirizzi:
Second Mil Med Univ, Dept Neurobiol, Shanghai 200433, Peoples R China Second Mil Med Univ Shanghai Peoples R China 200433 433, Peoples R China
Titolo Testata:
NEUROSCIENCE LETTERS
fascicolo: 1, volume: 300, anno: 2001,
pagine: 54 - 58
SICI:
0304-3940(20010302)300:1<54:CMTIOR>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
WITHDRAWAL EXCITATION; MAGNOCELLULAR NEURONS; ORGANOTYPIC CULTURES; OPIOID INHIBITION; DEPENDENCE; PATHWAYS; NALOXONE; TOLERANT;
Keywords:
organotypic slice culture; oxytocin neuron; supraoptic nucleus; mu-opioid receptor; morphine dependence; colocalization; radioimmunoassay; in situ hybridization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: You, ZD Second Mil Med Univ, Dept Neurobiol, 800 Xiangyin Rd, Shanghai 200433, Peoples R China Second Mil Med Univ 800 Xiangyin Rd Shanghai Peoples R China 200433
Citazione:
J.H. Li et al., "Chronic morphine treatment inhibits oxytocin release from the supraoptic nucleus slices of rats", NEUROSCI L, 300(1), 2001, pp. 54-58

Abstract

Effect of chronic morphine treatment on oxytocin (OT) release from the long term-cultured organotypic slice of the supraoptic nucleus (SON) was investigated using radioimmunoassay. The co-localization of oxytocin and mu-opioid receptor in neurons within the SON was observed with the double-labeled methods of in situ hybridization combined with immunohistochemistry. After exposure to morphine for 6 days, the OT levels in culture media were significantly decreased. Naloxone caused much greater release of OT in chronic morphine treatment group than in controls. Naloxone has no effect after acutemorphine treatment. 90% of OT-ir (immunoreactive) neurons expressed mu -opioid receptor mRNA in the SON and 45% of the neurons that expressed CL-opioid receptor mRNAs were OT-ir neurons. These results indicated that the neurons within SON could develop dependence on morphine in vitro, and these effects might be exerted via mu -opioid receptor in oxytocin neurons of the SON. (C) 2001 Published by Elsevier Science Ireland Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/08/20 alle ore 08:28:49