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Titolo:
CHARACTERIZATION OF THE MURINE BETA-HEXOSAMINIDASE (HEXB) GENE
Autore:
TRIGGSRAINE BL; BENOIT G; SALO TJ; TRASLER JM; GRAVEL RA;
Indirizzi:
UNIV MANITOBA,DEPT BIOCHEM & MOLEC BIOL,770 BANNATYNE AVE WINNIPEG R3E 0W3 MB CANADA MCGILL UNIV,MONTREAL CHILDRENS HOSP,RES INST MONTREAL H3H 1P3 PQ CANADA MCGILL UNIV,DEPT PEDIAT MONTREAL H3A 2T5 PQ CANADA MCGILL UNIV,DEPT PHARMACOL & THERAPEUT MONTREAL PQ CANADA MCGILL UNIV,DEPT HUMAN GENET MONTREAL PQ CANADA
Titolo Testata:
Biochimica et biophysica acta. Molecular basis of disease
fascicolo: 1-2, volume: 1227, anno: 1994,
pagine: 79 - 86
SICI:
0925-4439(1994)1227:1-2<79:COTMB(>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALPHA-CHAIN; N-ACETYLHEXOSAMINIDASE; TAY-SACHS; EXTENSIVE HOMOLOGY; HUMAN-CHROMOSOMES; SEQUENCE-ANALYSIS; LYSOSOMAL-ENZYME; SUBUNIT; CLONES; ASSIGNMENT;
Keywords:
G(M2) GANGLIOSIDOSIS; REGULATION; PROMOTER; MESSENGER-RNA QUANTITATION; (MOUSE);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
B.L. Triggsraine et al., "CHARACTERIZATION OF THE MURINE BETA-HEXOSAMINIDASE (HEXB) GENE", Biochimica et biophysica acta. Molecular basis of disease, 1227(1-2), 1994, pp. 79-86

Abstract

The murine HEXB gene, encoding the beta-subunit of the lysosomal hydrolase, beta-hexosaminidase, was isolated from a mouse cosmid library as a single cosmid clone. The entire gene spans 22 kb, considerably less than the 40 kb spanned by its human counterpart. it is highly homologous to the human gene. The 14 intron-exon junctions are entirely conserved, although the intron sequences diverge rapidly. Upstream of the coding region, a 1.3 kb segment was sequenced and shown to function asa promoter when fused with a reporter gene and expressed in monkey COS-7 cells. A short sequence (100 bp), near the start of the coding region, exhibits strong homology to the human HEXB promoter. Analysis of the tissue distribution of the HEXB mRNA in 129/Sv male mice revealed up to 28-fold tissue-specific variations in transcript levels. The kidney and the epididymis had the highest mRNA levels consistent with past surveys of enzyme activity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 13:42:31