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Titolo:
Mutagenic specificity of the base analog 6-N-hydroxylaminopurine in the LYS2 gene of yeast Saccharomyces cerevisiae
Autore:
Kulikov, VV; Derkatch, IL; Noskov, VN; Tarunina, OV; Chernoff, YO; Rogozin, IB; Pavlov, YI;
Indirizzi:
Sankt Petersburg State Univ, Dept Genet, St Petersburg 199034, Russia Sankt Petersburg State Univ St Petersburg Russia 199034 g 199034, Russia Georgia Inst Technol, Sch Biol, Atlanta, GA 30332 USA Georgia Inst Technol Atlanta GA USA 30332 Sch Biol, Atlanta, GA 30332 USA Russian Acad Sci, Siberian Branch, Inst Cytol & Genet, Novosibirsk, RussiaRussian Acad Sci Novosibirsk Russia Cytol & Genet, Novosibirsk, Russia
Titolo Testata:
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS
fascicolo: 2, volume: 473, anno: 2001,
pagine: 151 - 161
SICI:
1386-1964(20010220)473:2<151:MSOTBA>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA-REPLICATION ERRORS; ESCHERICHIA-COLI; POLYMERASE-EPSILON; MUTATION-RATES; LAC REPRESSOR; CAN1 LOCUS; REPAIR; TRANSLATION; DELTA; TERMINATION;
Keywords:
base analog mutagenesis; yeast; suppression; prions; mutation hotspots;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Pavlov, YI NIEHS, Labs Mol Genet & Struct Biol, POB 12233, Res Triangle Pk, NC 27709 USA NIEHS POB 12233 Res Triangle Pk NC USA 27709 e Pk, NC 27709 USA
Citazione:
V.V. Kulikov et al., "Mutagenic specificity of the base analog 6-N-hydroxylaminopurine in the LYS2 gene of yeast Saccharomyces cerevisiae", MUT RES-F M, 473(2), 2001, pp. 151-161

Abstract

We used the LYS2 gene mutational system to study mutation specificity of the base analog 6-N-hydroxylaminopurine (HAP) in yeast. We characterized phenotypes of mutations using codon-specific nonsense suppressors and the testemploying inactivation of the release factor Sup35 due to overexpression and formation of prion-like derivative [PSI]. We have shown that HAP inducespredominantly nonsense mutations. While the tests using codon-specific nonsense-suppressors allowed to identify only about 50% of nonsense-mutations,all the nonsense-mutations were identified in the test with defective Sup35. We determined and analyzed the spectrum of MAP-induced nucleotide changes in two regions of the gene. HAP induces predominantly GC --> AT transitions in a hotspots of a central position of trinucleotide GGA or AGG. Directionality of these transitions is consistent with the idea that initial dHAPMP incorporation in the leading strand is more genetically dangerous than inlagging DNA strand. We revealed a specific context inhibitory for HAP mutagenesis, a "T" in -1 position to mutation site. (C) 2001 Elsevier Science B. V. All rights reserved.

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Documento generato il 02/04/20 alle ore 19:23:25