Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Regulation of the retinoblastoma-dependent Mdm2 and E2F-1 signaling pathways during neuronal apoptosis
Autore:
Trinh, E; Boutillier, AL; Loeffler, JP;
Indirizzi:
CNRS 21, UMR 7519, F-67084 Strasbourg, France CNRS 21 Strasbourg France F-67084 , UMR 7519, F-67084 Strasbourg, France
Titolo Testata:
MOLECULAR AND CELLULAR NEUROSCIENCE
fascicolo: 2, volume: 17, anno: 2001,
pagine: 342 - 353
SICI:
1044-7431(200102)17:2<342:ROTRMA>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
CEREBELLAR GRANULE NEURONS; S-PHASE ENTRY; UBIQUITIN-PROTEASOME PATHWAY; TRANSCRIPTION FACTOR E2F-1; ICE-LIKE PROTEASE; CELL-DEATH; CYCLIN D1; POSTMITOTIC NEURONS; DNA FRAGMENTATION; FAMILY PROTEINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
71
Recensione:
Indirizzi per estratti:
Indirizzo: Boutillier, AL CNRS 21, UMR 7519, Rue Rene Descartes, F-67084 Strasbourg, France CNRS 21 Rue Rene Descartes Strasbourg France F-67084 rance
Citazione:
E. Trinh et al., "Regulation of the retinoblastoma-dependent Mdm2 and E2F-1 signaling pathways during neuronal apoptosis", MOL CELL NE, 17(2), 2001, pp. 342-353

Abstract

We have previously demonstrated that the apoptotic signaling pathway in K+-deprived cerebellar granule neurons involves a caspase-dependent cleavage of the retinoblastoma protein (Rb). Here, we have further investigated the functional consequences of this cleavage on two Rb-binding partners: the oncoprotein Mdm2 and the transcription factor E2F-1. A K+ deprivation time course leads to a caspase inhibitor-sensitive degradation of Mdm2. Experimental blockade of Mdm2 expression with antisense oligodeoxynucleotides (ODN) results in neuronal death, suggesting an active role of Mdm2 in neuroprotection. By contrast, the E2F-1 protein accumulates in a caspase-independent manner following K+ withdrawal, a consequence of increased gene transcription. This is likely to result from the rapid cyclin-dependent kinase 4 activation observed in LK, that correlates with a transient Rb phosphorylation. Counteracting E2F-1 upregulation with antisense ODNs prevents neuronal loss. Taken together, these data demonstrate that Rb is a central player in regulating both caspase-dependent and -independent events leading to apoptosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/10/20 alle ore 02:04:29