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Titolo:
Differential expression of mycobacterial proteins following phagocytosis by macrophages
Autore:
Monahan, IM; Betts, J; Banerjee, DK; Butcher, PD;
Indirizzi:
Univ London St Georges Hosp, Sch Med, Dept Med Microbiol, London SW17 0RE,England Univ London St Georges Hosp London England SW17 0RE don SW17 0RE,England Glaxo Wellcome Res & Dev Ltd, Resp Syst, Med Res Ctr, Stevenage SG1 2NY, Herts, England Glaxo Wellcome Res & Dev Ltd Stevenage Herts England SG1 2NYrts, England
Titolo Testata:
MICROBIOLOGY-UK
, volume: 147, anno: 2001,
parte:, 2
pagine: 459 - 471
SICI:
1350-0872(200102)147:<459:DEOMPF>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEAT-SHOCK PROTEINS; POLYACRYLAMIDE GELS; BOVIS BCG; SALMONELLA-TYPHIMURIUM; BACTERIAL-INFECTIONS; MASS-SPECTROMETRY; GENE-EXPRESSION; TUBERCULOSIS; VIRULENCE; CELLS;
Keywords:
mycobacteria; gene expression; macrophages; proteomics; tuberculosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
61
Recensione:
Indirizzi per estratti:
Indirizzo: Butcher, PD Univ London St Georges Hosp, Sch Med, Dept Med Microbiol, Cranmer Terrace,London SW17 0RE, England Univ London St Georges Hosp Cranmer Terrace London England SW17 0RE
Citazione:
I.M. Monahan et al., "Differential expression of mycobacterial proteins following phagocytosis by macrophages", MICROBIO-UK, 147, 2001, pp. 459-471

Abstract

Mycobacterium tuberculosis resides within the macrophages of the host, butthe molecular and cellular mechanisms of survival are poorly understood. Recent evidence suggests that the attenuated vaccine strain Mycobacterium bovis BCG is both a deletion and regulatory mutant, yet retains both its immunoprotective and intra-macrophage survival potential. In an attempt to define M. bovis BCG genes expressed during interaction with macrophages, the patterns of protein synthesis were examined by both one- and two-dimensional gel electrophoresis of BCC while inside the human leukaemic macrophage cellline THP-1. This study demonstrated that BCC expresses proteins while resident inside macrophages that are not expressed during in vitro growth in culture media or under conditions of heat shock. Western blotting analysis revealed that some of the differentially expressed proteins are specifically recognized by human M, tuberculosis-infected sera. Proteome analysis by two-dimensional electrophoresis and MS identified six abundant proteins that showed increased expression inside macrophages: 16 kDa a-crystallin (HspX), GroEL-1 and GroEL-2, a 31.7 kDa hypothetical protein (Rv2623), InhA and elongation factor Tu (Tuf), Identification of proteins by such a strategy willhelp elucidate the molecular basis of the attenuation and the vaccine potential of BCG, and may provide antigens that distinguish infection with M, tuberculosis from vaccination with BCG.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 08:48:18