Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Comparison of toxinotyping and PCR ribotyping of Clostridium difficile strains and description of novel toxinotypes
Autore:
Rupnik, M; Brazier, JS; Duerden, BI; Grabnar, M; Stubbs, SLJ;
Indirizzi:
Univ Ljubljana, Dept Biol, Ljubljana 1000, Slovenia Univ Ljubljana Ljubljana Slovenia 1000 pt Biol, Ljubljana 1000, Slovenia Univ Wales Hosp, Publ Hlth Lab, Anaerobe Reference Unit, Cardiff CF4 4XW, S Glam, Wales Univ Wales Hosp Cardiff S Glam Wales CF4 4XW rdiff CF4 4XW, S Glam, Wales
Titolo Testata:
MICROBIOLOGY-UK
, volume: 147, anno: 2001,
parte:, 2
pagine: 439 - 447
SICI:
1350-0872(200102)147:<439:COTAPR>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYACRYLAMIDE-GEL ELECTROPHORESIS; B-POSITIVE STRAIN; TOXIN-A; PATHOGENICITY LOCUS; TYPING SCHEME; SEROGROUP-F; GLYCOSYLTRANSFERASES; TRANSCRIPTION; REGION;
Keywords:
Clostridium difficile; toxinotyping; PCR ribotypes; tcdA(+)B(-) strains; variant toxin genes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Rupnik, M Univ Ljubljana, Dept Biol, Vecna Pot 111, Ljubljana 1000, Slovenia Univ Ljubljana Vecna Pot 111 Ljubljana Slovenia 1000 , Slovenia
Citazione:
M. Rupnik et al., "Comparison of toxinotyping and PCR ribotyping of Clostridium difficile strains and description of novel toxinotypes", MICROBIO-UK, 147, 2001, pp. 439-447

Abstract

Toxinotyping and PCR ribotyping are two methods that have been used to type Clostridium difficile isolates. Toxinotyping is based on PCR-RFLP analysis of a 19 kb region encompassing the C. difficile pathogenicity locus. PCR ribotyping is based on comparison of patterns of PCR products of the 165-23S rRNA intergenic spacer region. Representative strains (101) from a C. difficile PCR ribotype library and 22 strains from previously described toxinotypes were analysed to compare ribotyping with toxinotyping. Within this panel of strains all 11 toxinotypes (0-X) described previously and an additional 5 novel toxinotypes (XI-XV) were observed. PCR ribotyping and toxinotyping correlated well and usually all strains within a given ribotype had similar changes in toxin genes. The new toxinotype XI comprises strains that did not express toxins TcdA or TcdB at detectable levels, but contained partof the tcdA gene. Strains of toxinotype XII exhibit changes only in the 5'end of the tcdB gene. Toxinotype XIV is composed of strains that have a large insertion at the beginning of the tcdA gene. A total of 25 of the 89 tested PCR ribotypes of C. difficile contained variant strains. It was estimated that they represent 7.7% of the total number of strains in the AnaerobeReference Unit collection.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 15:21:42