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Titolo:
A cGMP-dependent protein kinase is implicated in wild-type motility in C-elegans
Autore:
Stansberry, J; Baude, EJ; Taylor, MK; Chen, PJ; Jin, SW; Ellis, RE; Uhler, MD;
Indirizzi:
Univ Michigan, Dept Biol Chem, Ann Arbor, MI 48104 USA Univ Michigan Ann Arbor MI USA 48104 t Biol Chem, Ann Arbor, MI 48104 USA Univ Michigan, Grad Program Neurosci, Ann Arbor, MI 48104 USA Univ Michigan Ann Arbor MI USA 48104 am Neurosci, Ann Arbor, MI 48104 USA Univ Michigan, Dept Biol, Ann Arbor, MI 48104 USA Univ Michigan Ann ArborMI USA 48104 , Dept Biol, Ann Arbor, MI 48104 USA Univ Michigan, Mental Hlth Res Inst, Ann Arbor, MI 48104 USA Univ Michigan Ann Arbor MI USA 48104 th Res Inst, Ann Arbor, MI 48104 USA
Titolo Testata:
JOURNAL OF NEUROCHEMISTRY
fascicolo: 4, volume: 76, anno: 2001,
pagine: 1177 - 1187
SICI:
0022-3042(200102)76:4<1177:ACPKII>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
I-DEFICIENT MICE; CAENORHABDITIS-ELEGANS; CYCLIC-GMP; SMOOTH-MUSCLE; BOVINE AORTA; GUANYLATE CYCLASE; MOLECULAR-CLONING; SENSORY NEURONS; PHOSPHORYLATION; RAT;
Keywords:
C. elegans; cGMP; cGMP-dependent protein kinase; neuronal; motility phosphorylation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
69
Recensione:
Indirizzi per estratti:
Indirizzo: Uhler, MD Univ Michigan, Dept Biol Chem, Neurosci Labs Bldg,1103 E Huron St, Ann Arbor, MI 48104 USA Univ Michigan Neurosci Labs Bldg,1103 E Huron StAnn Arbor MI USA 48104
Citazione:
J. Stansberry et al., "A cGMP-dependent protein kinase is implicated in wild-type motility in C-elegans", J NEUROCHEM, 76(4), 2001, pp. 1177-1187

Abstract

In mammals, cyclic GMP and cGMP-dependent protein kinases (cGKs) have beenimplicated in the regulation of many neuronal functions including long-term potentiation and long-term depression of synaptic efficacy. To develop Caenorhabditis elegans as a model system for studying the neuronal function of the cGKs, we cloned and characterized the cgk-1 gene. A combination of approaches showed that cgk-1 produces three transcripts, which differ in their first exon but are similar in length. Northern analysis of C. elegans RNA, performed with a probe designed to hybridize to all three transcripts, confirmed that a major 3.0 kb cgk-1 transcript is present at all stages of development. To determine if the CGK-1C protein was a cGMP-dependent protein kinase, CGK-1C was expressed in S/9 cells and purified. CGK-1C shows a K-a of 190 +/- 14 nM for cGMP and 18.4 +/- 2 muM for cAMP. Furthermore, CGK-1C undergoes autophosphorylation in a cGMP-dependent manner and is inhibited by the commonly used cGK inhibitor, KT5823. To determine which cells expressed CGK-1C, a 2.4-kb DNA fragment from the promoter of CGK-1C was used to drive GFP expression. The CGK-1C reporter construct is strongly expressed in the ventral nerve cord and in several other neurons as well as the marginalcells of the pharynx and intestine. Finally, RNA-mediated interference of CGK-1 resulted in movement defects in nematode larvae. These results provide the first demonstration that cGMP-dependent protein kinase is present in neurons of C.elegans and show that this kinase is required for normal motility.

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Documento generato il 09/07/20 alle ore 13:46:22