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Titolo:
The effects of stimulating protease-activated receptor-1 and-2 in A172 human glioblastoma
Autore:
Okamoto, T; Nishibori, M; Sawada, K; Iwagaki, H; Nakaya, N; Jikuhara, A; Tanaka, N; Saeki, K;
Indirizzi:
Okayama Univ, Dept Pharmacol, Sch Med, Okayama 7008558, Japan Okayama Univ Okayama Japan 7008558 acol, Sch Med, Okayama 7008558, Japan Okayama Univ, Dept Surg 1, Sch Med, Okayama, Japan Okayama Univ Okayama Japan a Univ, Dept Surg 1, Sch Med, Okayama, Japan
Titolo Testata:
JOURNAL OF NEURAL TRANSMISSION
fascicolo: 2, volume: 108, anno: 2001,
pagine: 125 - 140
SICI:
0300-9564(2001)108:2<125:TEOSPR>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTHROMBIN MESSENGER-RNA; THROMBIN RECEPTOR; MOLECULAR-CLONING; PLATELET ACTIVATION; ENDOTHELIAL-CELLS; NEURITE OUTGROWTH; CROSS-REACTIVITY; NERVOUS-SYSTEM; HUMAN BRAIN; KINASE-C;
Keywords:
protease-activated receptor; A172 glioblastoma; calcium signal; proliferation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Okamoto, T Okayama Univ, Dept Pharmacol, Sch Med, 2-5-1 Shikata Cho, Okayama 7008558,Japan Okayama Univ 2-5-1 Shikata Cho Okayama Japan 7008558 558,Japan
Citazione:
T. Okamoto et al., "The effects of stimulating protease-activated receptor-1 and-2 in A172 human glioblastoma", J NEURAL TR, 108(2), 2001, pp. 125-140

Abstract

Human glioblastoma cell line A172 expressed protease-activated receptor-1 and -2 (PAR-1 and PAR-2). We investigated the effects of the stimulation ofthese receptors by receptor-activating agonist peptides on the Ca2+ signaling, protein kinase C translocation, cell morphology and cell proliferationin A172. Both PAR-1 agonist SFLLRN and PAR-2 agonist SLIGKV induced an increase in [Ca2+]i. The prior treatment of A172 with PAR-2 agonist SLIGKV didnot influence the [Ca2+]i response to PAR-1 agonist SFLLRN or thrombin, however, the prior treatment with PAR-1 agonist SFLLRN or thrombin completelyabolished the second response to PAR-2 agonist SLIGKV. Treatment with eachagonist peptide produced thinner and fewer processes in A172. The PAR-2 agonist inhibited the proliferation of A172 significantly while PAR-1 agonistdid not. PKC-alpha and gamma were translocated from cytosol to membrane with either PAR-1 or PAR-2 stimulation, however, iota was specifically translocated with SFLLRN, and lambda with SLIGKV, respectively. These results indicated that PAR-1 and PAR-2 stimulation produced a similar [Ca2+]i responseand morphological changes in A172 glioblastoma while the effects on the cell proliferation and activation of PKC isozymes were distinct, suggesting that different signal transduction pathways were activated by these receptors. The uni-directional cross desensitization implies a functional linkage between PAR-1 and PAR-2 receptors.

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Documento generato il 26/11/20 alle ore 11:15:47