Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Binding site of acyl moiety in ester hydrolysis by Candida rugosa lipase
Autore:
Goto, M; Kawasaki, M; Kometani, T; Nonaka, T; Mitsui, Y;
Indirizzi:
Toyama Natl Coll Technol, Dept Chem & Biochem Engn, Toyama 9398630, Japan Toyama Natl Coll Technol Toyama Japan 9398630 ngn, Toyama 9398630, Japan Toyama Prefectural Univ, Fac Engn, Toyama 9390398, Japan Toyama Prefectural Univ Toyama Japan 9390398 Engn, Toyama 9390398, Japan Nagaoka Univ Technol, Fac Engn, Nagaoka, Niigata 9402188, Japan Nagaoka Univ Technol Nagaoka Niigata Japan 9402188 Niigata 9402188, Japan
Titolo Testata:
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
fascicolo: 4-6, volume: 11, anno: 2001,
pagine: 1029 - 1033
SICI:
1381-1177(20010122)11:4-6<1029:BSOAMI>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
CARBOXYLIC-ACIDS; STRUCTURAL BASIS; ENANTIOSELECTIVITY; ESTERIFICATION; RESOLUTION; ALCOHOLS; DONOR; RULE;
Keywords:
Candida rugosa lipase; specific activity; hydrolysis; tunnel structure; p-nitrophenyl ester;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Goto, M Toyama Natl Coll Technol, Dept Chem & Biochem Engn, Hongo 13, Toyama 9398630, Japan Toyama Natl Coll Technol Hongo 13 Toyama Japan 9398630 630, Japan
Citazione:
M. Goto et al., "Binding site of acyl moiety in ester hydrolysis by Candida rugosa lipase", J MOL CAT B, 11(4-6), 2001, pp. 1029-1033

Abstract

The sizes of the large, medium, and small substituent recognition sites (L, M, and S pockets, respectively) in Candida rugosa lipase (CRL) were roughly estimated by measuring the specific hydrolytic activity against several p-nitrophenyl esters. These relative sizes were assessed as L pocket > phenyl, ethyl > M pocket > methyl > S pocket > hydrogen. The hydrolysis of a series of p-nitrophenyl esters of omega -substituted fatty acids was also examined. In this series, p-nitrophenyl esters having one methylene between the ester-carbonyl carbon and cyclohexyl, phenyl, or isopropyl moiety largelydemonstrated decreases in hydrolytic activity. (C) 2001 Elsevier Science B. V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 07:48:44