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Titolo:
Small water amounts increase the catalytic behaviour of polar organic solvents pre-treated Candida rugosa lipase
Autore:
Chamorro, S; Alcantara, AR; de la Casa, RM; Sinisterra, JV; Sanchez-Montero, JM;
Indirizzi:
Univ Complutense Madrid, Fac Farm, Dept Quim Organ & Farmaceut, E-28040 Madrid, Spain Univ Complutense Madrid Madrid Spain E-28040 ceut, E-28040 Madrid, Spain
Titolo Testata:
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
fascicolo: 4-6, volume: 11, anno: 2001,
pagine: 939 - 947
SICI:
1381-1177(20010122)11:4-6<939:SWAITC>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENANTIOSELECTIVITY; CYLINDRACEA; ISOENZYMES; ESTERS; MEDIA; PURIFICATION; HYDROLYSIS; DEPENDENCE; HYDRATION; ISOTHERM;
Keywords:
Candida rugosa lipase; esterification; ketoprofen; organic solvents; ammonium sulphate; water activity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Sanchez-Montero, JM Univ Complutense Madrid, Fac Farm, Dept Quim Organ & Farmaceut, E-28040 Madrid, Spain Univ Complutense Madrid Madrid Spain E-28040 , Spain
Citazione:
S. Chamorro et al., "Small water amounts increase the catalytic behaviour of polar organic solvents pre-treated Candida rugosa lipase", J MOL CAT B, 11(4-6), 2001, pp. 939-947

Abstract

Following a simple and quick treatment based on dissolving the crude lipase of Candida rugosa in different percentages (v/v) of several polar organicsolvents (methanol, ethanol, acetone, l-propanol, 2-propanol, l-butanol and 2-butanol), and a further dialysis, different preparations with enhanced activity and thermal stability were obtained. This improved catalytic behaviour was observed in aqueous media (more efficient triglyceride hydrolysis)as well as in anhydrous organic solvents (better enantioselectivity and/oryield in the esterification of R- and S-ketoprofen). Combination of this treatment with organic solvents with a correct controlof water activity in the reaction media allowed a much better catalytic behaviour, dramatically increasing the reaction yield and the enantiodiscrimination ability in the esterification of S-ketoprofen vs. the R counterpart. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 13:48:25