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Titolo:
The use of a solid adsorber resin for enrichment of bacteria with toxic substrates and to identify metabolites: degradation of naphthalene, o-, and m-xylene by sulfate-reducing bacteria
Autore:
Morasch, B; Annweiler, E; Warthmann, RJ; Meckenstock, RU;
Indirizzi:
Univ Konstanz, Lehrstuhl Mikrobiol Okol, D-78457 Constance, Germany Univ Konstanz Constance Germany D-78457 Okol, D-78457 Constance, Germany Univ Hamburg, Inst Biogeochem & Meereschem, D-20146 Hamburg, Germany Univ Hamburg Hamburg Germany D-20146 eereschem, D-20146 Hamburg, Germany
Titolo Testata:
JOURNAL OF MICROBIOLOGICAL METHODS
fascicolo: 2, volume: 44, anno: 2001,
pagine: 183 - 191
SICI:
0167-7012(20010301)44:2<183:TUOASA>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYCYCLIC AROMATIC-HYDROCARBONS; ANAEROBIC DEGRADATION; DENITRIFYING BACTERIUM; METHANOGENIC CONSORTIUM; ENZYMATIC REDUCTION; INITIAL REACTIONS; KEY REACTION; BENZOYL-COA; STRAIN-T; TOLUENE;
Keywords:
aromatic hydrocarbons; anaerobic degradation; bioremediation; BTEX; PAH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Meckenstock, RU Univ Konstanz, Lehrstuhl Mikrobiol Okol, Univ Str 10, D-78457 Constance, Germany Univ Konstanz Univ Str 10 Constance Germany D-78457rmany
Citazione:
B. Morasch et al., "The use of a solid adsorber resin for enrichment of bacteria with toxic substrates and to identify metabolites: degradation of naphthalene, o-, and m-xylene by sulfate-reducing bacteria", J MICROB M, 44(2), 2001, pp. 183-191

Abstract

Anaerobic sulfate-reducing bacteria were enriched from contaminated aquifer samples with naphthalene, o-, and m-xylene as sole carbon and energy source in the presence of Amberlite-XAD7, a solid adsorber resin. XAD7 served as a substrate reservoir maintaining a constantly low substrate concentration in the culture medium. In equilibration experiments with XAD7, the aromatic hydrocarbons needed up to 5 days to achieve equilibrium between the water and the XAD7 phase. The equilibrium concentration was directly correlatedwith the amount of added substrate and XAD7. In the enrichments presented here, XAD7 and aromatic hydrocarbons were adjusted to maintain substrate concentrations of 100 muM m-, or o-xylene, or 50 muM naphthalene. After five subsequent transfers. the three cultures were able to grow with higher substrate concentrations in the absence of XAD7 although they grew best with lower hydrocarbon concentrations. Two new xylene-degrading cultures were obtained that could not utilise toluene as carbon source. O-xylene was degradedanaerobically by a culture, which could also oxidise in-xylene but not p-xylene. Eighty-three percent of the electrons from o-xylene oxidation were recovered in the produced sulfide, indicating a complete oxidation to CO2. Another sulfate-reducing enrichment culture oxidised m-xylene completely to CO2 but not o-, or p-xylene. A naphthalene-degrading sulfate-reducing enrichment culture oxidised naphthalene completely to CO2. Metabolites of naphthalene degradation were recovered from the XAD7 phase and subjected to GC/Msanalysis. Besides the metabolites 2-naphthoic acid and decahydro-2-naphthoic acid which were identified by the mass spectrum and coelution with chemically synthesised reference compounds, the reduced 2-naphthoic acid derivatives 5,6,7,8-tetrahydro-2-naphthoic acid and octahydro-2-naphthoic acid were tentatively identified by their mass spectra. Cultivation of bacterial cultures in the presence of XAD7 and subsequent derivatisation and extractionof metabolites directly from the solid XAD7 resin provides a new method for the isolation of sensitive bacteria and identification of metabolites. (C) 2001 Published by Elsevier Science B.V.

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Documento generato il 07/07/20 alle ore 21:29:36