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Titolo:
Genetic and ultrastructural characterization of a European isolate of the fatal endotheliotropic elephant herpesvirus
Autore:
Ehlers, B; Burkhardt, S; Goltz, M; Bergmann, V; Ochs, A; Weiler, H; Hentschke, J;
Indirizzi:
Robert Koch Inst, D-13353 Berlin, Germany Robert Koch Inst Berlin Germany D-13353 ch Inst, D-13353 Berlin, Germany Inst Lebensmittel Arzneimittel & Tierrseuchen, D-10557 Berlin, Germany Inst Lebensmittel Arzneimittel & Tierrseuchen Berlin Germany D-10557 any Free Univ Berlin, Inst Vet Pathol, D-14163 Berlin, Germany Free Univ Berlin Berlin Germany D-14163 Pathol, D-14163 Berlin, Germany Zool Garten Berlin AG, D-10787 Berlin, Germany Zool Garten Berlin AG Berlin Germany D-10787 AG, D-10787 Berlin, Germany
Titolo Testata:
JOURNAL OF GENERAL VIROLOGY
, volume: 82, anno: 2001,
parte:, 3
pagine: 475 - 482
SICI:
0022-1317(200103)82:<475:GAUCOA>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEPENDENT DNA-POLYMERASES; GLYCOPROTEIN-B; SEQUENCE; GENOME;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Ehlers, B Robert Koch Inst, Nordufer 20, D-13353 Berlin, Germany Robert Koch Inst Nordufer 20 Berlin Germany D-13353 in, Germany
Citazione:
B. Ehlers et al., "Genetic and ultrastructural characterization of a European isolate of the fatal endotheliotropic elephant herpesvirus", J GEN VIROL, 82, 2001, pp. 475-482

Abstract

A male Asian elephant (Elephas maximus) died at the Berlin zoological gardens in August 1998 of systemic infection with the novel endotheliotropic elephant herpesvirus (EIHV-1). This virus causes a fatal haemorrhagic diseasein Asian elephants, the so-called endothelial inclusion body disease, as reported from North American zoological gardens. In the present work, EIHV-1was visualized ultrastructurally in affected organ material. Furthermore, a gene block comprising the complete glycoprotein B (gB) and DNA polymerase(DPOL) genes as well as two partial genes was amplified by PCR-based genome walking and sequenced. The gene content and arrangement were similar to those of members of the Betaherpesvirinae. However, phylogenetic analysis with gB and DPOL consistently revealed a very distant relationship to the betaherpesviruses. Therefore, EIHV-1 may be a member of a new genus or even a new herpesvirus subfamily, The sequence information generated was used to set up a nested-PCR assay for diagnosis of suspected cases of endothelial inclusion body disease. Furthermore, it will aid in the development of antibody-based detection methods and of vaccination strategies against this fatalherpesvirus infection in the endangered Asian elephant.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 00:55:57