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Titolo:
Growth inhibition and apoptosis of gastric cancer cell lines by Anemarrhena asphodeloides Bunge
Autore:
Takeda, Y; Togashi, H; Matsuo, T; Shinzawa, H; Takeda, Y; Takahashi, T;
Indirizzi:
Yamagata Univ, Sch Med, Dept Internal Med 2, Yamagata 9909585, Japan Yamagata Univ Yamagata Japan 9909585 rnal Med 2, Yamagata 9909585, Japan
Titolo Testata:
JOURNAL OF GASTROENTEROLOGY
fascicolo: 2, volume: 36, anno: 2001,
pagine: 79 - 90
SICI:
0944-1174(200102)36:2<79:GIAAOG>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
FAS-MEDIATED APOPTOSIS; CYTOCHROME-C; ICE/CED-3 PROTEASE; FACTOR-BETA; ICE-LIKE; CARCINOMA; P53; EXPRESSION; INDUCTION; REQUIREMENT;
Keywords:
Anemarrhena asphodeloides Bunge; gastric cancer; apoptosis; caspase 3; cytochrome c;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Takeda, Y Yamagata Univ, Sch Med, Dept Internal Med 2, 2-2-2 Iidanishi, Yamagata 9909585, Japan Yamagata Univ 2-2-2 Iidanishi Yamagata Japan 9909585585, Japan
Citazione:
Y. Takeda et al., "Growth inhibition and apoptosis of gastric cancer cell lines by Anemarrhena asphodeloides Bunge", J GASTRO, 36(2), 2001, pp. 79-90

Abstract

In this study, we aimed to determine the growth inhibition and the induction of apoptotic cell death brought about by the herb Anemarrhena asphodeloides Bunge in gastric cancer cell lines, and to clarify the mechanism of this apoptosis. Water-soluble ingredients of A. asphodeloides, and the gastriccancer cell lines, MKN45 and KATO-III, were used in vitro. Growth inhibition, induction of cell death, morphological features, the presence of DNA ladders, increases in caspase-3-like activity, the effects of a caspase-3 inhibitor on apoptotic cell death, and the release of cytochrome c by A. asphodeloides were analyzed. A. asphodeloides inhibited the growth and decreasedthe viability of the gastric cancer cell lines. The viability of normal skin fibroblasts in the presence of low concentrations of A. asphodeloides was higher than that of gastric cancer cells. Apoptotic bodies and DNA ladders were observed to be induced in MKN45 and KATO-III by A. asphodeloides. The caspase 3 inhibitor, Ac-DEVD-CHO, inhibited the apoptotic cell death of gastric cancer cells induced by A. asphodeloides. The caspase 3-like activity in MKN45 and KATO-III cells increased after the addition of A. asphodeloides. Cytochrome c was released from mitochondria into the cytosol 8 h afterthe addition of A. asphodeloides, and reached a peak at 16 h. The peak of cytochrome c release was earlier than that of caspase 3-like activity. We concluded that A. asphodeloides inhibited the growth of the gastric cancer cell lines MKN45 and KATO-III and induced apoptosis. The apoptosis of MKN45 and KATO-III cells induced by A. asphodeloides was associated with the release of cytochrome c from the mitochondria, followed by an increase in caspase 3-like activity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/08/20 alle ore 09:06:37