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Titolo:
IFN-gamma-dependent transcription of MHC class IIIA is impaired in macrophages from aged mice
Autore:
Herrero, C; Marques, L; Lloberas, J; Celada, A;
Indirizzi:
Univ Barcelona, Fac Biol, Dept Fisiol Biol Macrofag, E-08028 Barcelona, Spain Univ Barcelona Barcelona Spain E-08028 acrofag, E-08028 Barcelona, Spain Univ Barcelona, Fundacio August Pi & Sunyer, E-08028 Barcelona, Spain UnivBarcelona Barcelona Spain E-08028 Sunyer, E-08028 Barcelona, Spain
Titolo Testata:
JOURNAL OF CLINICAL INVESTIGATION
fascicolo: 4, volume: 107, anno: 2001,
pagine: 485 - 493
SICI:
0021-9738(200102)107:4<485:ITOMCI>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAJOR HISTOCOMPATIBILITY COMPLEX; I-A-BETA; BARE LYMPHOCYTE SYNDROME; RECEPTOR EXPRESSION; DNA-BINDING; GENE; IDENTIFICATION; TRANSACTIVATOR; MOLECULES; PROMOTER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Celada, A Univ Barcelona, Fac Biol, Dept Fisiol Biol Macrofag, Avinguda Diagonal 645, E-08028 Barcelona, Spain Univ Barcelona Avinguda Diagonal 645 Barcelona Spain E-08028 in
Citazione:
C. Herrero et al., "IFN-gamma-dependent transcription of MHC class IIIA is impaired in macrophages from aged mice", J CLIN INV, 107(4), 2001, pp. 485-493

Abstract

To determine the effect of aging on IFN-gamma -induced MHC class II antigen expression, we produced bone marrow-derived macrophages in vitro. In these conditions, we analyzed the effect of aging on the genomic expression of macrophages without the influence of other cell types that may be affected by aging. Although macrophages from young and aged mice showed an identicaldegree of differentiation, after incubation with IFN-gamma, the expressionat the cell surface of the IA complex and the levels of IA beta protein and mRNA were lower in aged macrophages. Moreover, the transcription of the IA beta gene was impaired in aged macrophages, The amount of transcription factors that bound to the W and X, but not to the Y, boxes of the IA beta promoter gene was lower in aged macrophages. Similar levels of CIITA mRNA were found after IFN-gamma treatment of both young and aged macrophages. This shows that neither the initial cascade that starts after the interaction ofIFN-gamma with the receptor nor the second signals involved in the expression of CIITA are impaired in aged macrophages. These data indicate that aging is associated with low levels of MHC class II gene induction by IFN-gamma because of impaired transcription.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 11:40:07