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Titolo:
Construction of gene therapy vectors targeting thyroid cells: Enhancement of activity and specificity with histone deacetylase inhibitors and agents modulating the cyclic adenosine 3 ',5 '-monophosphate pathway and demonstration of activity in follicular and anaplastic thyroid carcinoma cells
Autore:
Kitazono, M; Chuman, Y; Aikou, T; Fojo, T;
Indirizzi:
Kagoshima Univ, Fac Med, Dept Surg 1, Kagoshima 8908520, Japan Kagoshima Univ Kagoshima Japan 8908520 Surg 1, Kagoshima 8908520, Japan NCI, Med Branch, DCS, NIH, Bethesda, MD 20892 USA NCI Bethesda MD USA 20892 I, Med Branch, DCS, NIH, Bethesda, MD 20892 USA
Titolo Testata:
JOURNAL OF CLINICAL ENDOCRINOLOGY AND METABOLISM
fascicolo: 2, volume: 86, anno: 2001,
pagine: 834 - 840
SICI:
0021-972X(200102)86:2<834:COGTVT>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
DOXORUBICIN PLUS CISPLATIN; VIRUS THYMIDINE KINASE; IN-VITRO; CANCER; LINES; EXPRESSION; PROMOTER; PAPILLARY; RETINOIDS; TRIAL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Kitazono, M Kagoshima Univ, Fac Med, Dept Surg 1, Sakuragaoka 8-35-1, Kagoshima 8908520, Japan Kagoshima Univ Sakuragaoka 8-35-1 Kagoshima Japan 8908520 pan
Citazione:
M. Kitazono et al., "Construction of gene therapy vectors targeting thyroid cells: Enhancement of activity and specificity with histone deacetylase inhibitors and agents modulating the cyclic adenosine 3 ',5 '-monophosphate pathway and demonstration of activity in follicular and anaplastic thyroid carcinoma cells", J CLIN END, 86(2), 2001, pp. 834-840

Abstract

Thyroid carcinoma accounts for the majority of deaths from endocrine cancers. Although effective therapies exist for well differentiated tumors, the treatment options for poorly differentiated and anaplastic tumors are much less effective. In the present study we demonstrate that the thyroglobulin (Tg) promoter can be used to direct specific expression of either luciferase or thymidine kinase in thyroid cancer cells. Furthermore, using a putative enhancer element for the Tg gene, the activity of the Tg promoter in and its specificity for thyroid cells were enhanced. In transient transfectantsor in stably transfected thyroid carcinoma cells, treatment with the histone deacetylase inhibitors, depsipeptide (FR9012228) and sodium butyrate, alone or in combination with 8-bromo-cAMP, resulted in further enhancement. In experiments in which the herpes simplex virus thymidine kinase (HSV-TK) gene was driven by the Tg promoter and the putative enhancer, HSV-TK expression and ganciclovir sensitivity were augmented. Similar results were obtained in two cell lines derived from a follicular thyroid carcinoma and in twoanaplastic thyroid carcinoma cell lines. In summary, we report the construction of a suicide HSV-TK vector with preferential toxicity for thyroid cells. The results in anaplastic thyroid carcinoma cells suggest that it may be of use in the full spectrum of thyroid malignancies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 03:51:24