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Titolo:
Ex vivo measurement of lipoprotein lipase-dependent very low density lipoprotein (VLDL)-triglyceride hydrolysis in human VLDL: An alternative to the postheparin assay of lipoprotein lipase activity?
Autore:
Pruneta, V; Autran, D; Ponsin, G; Marcais, C; Duvillard, L; Verges, B; Berthezene, F; Moulin, P;
Indirizzi:
Hop Antiquaille, Lab Metab Lipides, CNRS, ESA 5014, F-69005 Lyon, France Hop Antiquaille Lyon France F-69005 CNRS, ESA 5014, F-69005 Lyon, France Hop Antiquaille, Serv Endocrinol & Malad Nutr, F-69005 Lyon, France Hop Antiquaille Lyon France F-69005 l & Malad Nutr, F-69005 Lyon, France Ctr Hosp Lyon Sud, Biochim Lab, Lyon, France Ctr Hosp Lyon Sud Lyon France Hosp Lyon Sud, Biochim Lab, Lyon, France CHU Dijon, INSERM, U498, Serv Endocrinol Malad Metab, Dijon, France CHU Dijon Dijon France U498, Serv Endocrinol Malad Metab, Dijon, France
Titolo Testata:
JOURNAL OF CLINICAL ENDOCRINOLOGY AND METABOLISM
fascicolo: 2, volume: 86, anno: 2001,
pagine: 797 - 803
SICI:
0021-972X(200102)86:2<797:EVMOLL>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMA TRIGLYCERIDE LIPASES; FREE FATTY-ACIDS; QUANTITATIVE-DETERMINATION; IMMUNOCHEMICAL METHOD; SELECTIVE MEASUREMENT; HEPATIC LIPASE; HEPARIN; METABOLISM; GENE; HYPERTRIGLYCERIDEMIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Pruneta, V Hop Antiquaille, Lab Metab Lipides, CNRS, ESA 5014, 1 Rue Antiquaille, F-69005 Lyon, France Hop Antiquaille 1 Rue Antiquaille Lyon FranceF-69005 , France
Citazione:
V. Pruneta et al., "Ex vivo measurement of lipoprotein lipase-dependent very low density lipoprotein (VLDL)-triglyceride hydrolysis in human VLDL: An alternative to the postheparin assay of lipoprotein lipase activity?", J CLIN END, 86(2), 2001, pp. 797-803

Abstract

The plasma lipolysis of triglyceride (TG)-rich lipoproteins is mainly due to the activity of lipoprotein lipase (LPL). Albeit important for our analysis of certain physiopathological situations, the determination of the magnitude of LPL-dependent lipolysis is not easy to perform. This essentially results from the binding of LPL to the luminal surface of vascular endothelium. The measurements of the whole putative LPL activity have been achieved after injection of heparin, a procedure that releases LPL from endothelium. However, the physiopathological relevance of this postheparin lipolysis assay (PHLA) remains questionable because it has never been demonstrated thatthe bulk of endothelium-bound LPL was active. It has been recently shown that a small part of LPL is associated to circulating lipoproteins in nonheparinized plasma, raising the possibility that the lipolysis mediated by this circulating LPL might reflect the overall LPL-dependent TG hydrolysis in plasma. To address this question, we developeda new lipolysis assay in which the very low density lipoprotein (VLDL)-bound LPL-dependent VLDL-TG hydrolysis (LVTH) was directly determined through the measurement of nonesterified fatty acid (NEFA) release during in vitro incubations. LVTH measurements were performed in control subjects, in type 2 diabetics, and in either heterozygous or homozygous LPL-deficient patients. In the latter group, LVTH Values were extremely low. Those of heterozygous patients and of diabetics were similarly decreased by about 40% with respect to control group. Plasma TG concentrations exhibited an inverse relationship with LVTH level. In a subgroup of subjects, LVTH and PHLA were positively correlated and the inverse correlation of LVTH with plasma or VLDL-TGconcentration was stronger than that obtained with PHLA. To further study the validity of this new assay, we measured LVTH in nine subjects who were studied for their catabolism of VLDL labeled with stable isotope. No relation was observed between the direct hepatic removal of VLDL and LVTH, whereas the latter was strikingly correlated with the rate of conversion of VLDL to intermediary density lipoprotein. Collective consideration of these findings strongly suggests that LVTH is a physiologically relevant index which could advantageously replace the measurements of PHLA in numerous physiopathological situations.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 04:41:22