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Titolo:
Automated high-performance liquid chromatography method for the determination of rosiglitazone in human plasma
Autore:
Muxlow, AM; Fowles, S; Russell, P;
Indirizzi:
SmithKline Beecham Pharmaceut, Dept Drug Metab & Pharmacokinet, Welwyn Garden City AL6 9AR, Herts, England SmithKline Beecham Pharmaceut Welwyn Garden City Herts England AL6 9AR nd
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 752, anno: 2001,
pagine: 77 - 84
SICI:
1387-2273(20010305)752:1<77:AHLCMF>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
BIOLOGICAL SAMPLES PRIOR; SEQUENTIAL TRACE ENRICHMENT; DIALYSIS;
Keywords:
rosiglitazone;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Muxlow, AM SmithKline Beecham Pharmaceut, Dept Drug Metab & Pharmacokinet,The Frythe, Welwyn Garden City AL6 9AR, Herts, England SmithKline Beecham Pharmaceut The Frythe Welwyn Garden City Herts England AL6 9AR
Citazione:
A.M. Muxlow et al., "Automated high-performance liquid chromatography method for the determination of rosiglitazone in human plasma", J CHROMAT B, 752(1), 2001, pp. 77-84

Abstract

A robust, fully automated assay procedure for the determination of rosiglitazone (I, BRL-49653) in human plasma has been developed. Plasma concentrations of I were determined using automated sequential trace enrichment of dialysates (ASTED) coupled to reversed-phase high-performance liquid chromatography. Sequential automated dialysis of human plasma samples was followed by concentration of the dialysate by trace enrichment on a C-18 cartridge. Drug and internal standard, SE-204882 (II) were eluted from the trace enrichment cartridge by mobile phase (0.01 M ammonium acetate, pH 8-acetonitrile, 65:35, v/v) onto the HPLC column (a Novapak C-18, 4 mum, 100 x 5 mm radial compression cartridge) protected by a Guard-Pak C-18 cartridge. The compounds were detected by fluorescence detection, using an excitation wavelength of 247 nm, and emission wavelength of 367 nn. The lower limit of quantitation of the method was 3 ng/ml (200 mul aliquot) with linearity demonstrated up to 100 ng/ml. Within- and between-run precision and accuracy of determination were better than 10% across the calibration range. There was no evidence of instability of I in human plasma following three complete freeze-thaw cycles and samples can be safely stored for at least 7 months at -20 degreesC. This method has been successfully utilised to provide pharmacokinetic data throughout the clinical development of rosiglitazone. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/06/20 alle ore 11:29:51