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Titolo:
On-line, continuous and automatic monitoring of extracellular malondialdehyde concentration in anesthetized rat brain cortex
Autore:
Yang, CS; Tsai, PJ; Chen, WY; Kuo, JS;
Indirizzi:
Taichung Vet Gen Hosp, Dept Educ & Res, Taichung 407, Taiwan Taichung Vet Gen Hosp Taichung Taiwan 407 uc & Res, Taichung 407, Taiwan
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 752, anno: 2001,
pagine: 33 - 38
SICI:
1387-2273(20010305)752:1<33:OCAAMO>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; MEDIATED LIPID-PEROXIDATION; BIOLOGICAL-MATERIALS; ACID; INJURY; MALONALDEHYDE; HYDROPEROXIDES; MICRODIALYSIS; REPERFUSION;
Keywords:
on-line microdialysis; malondialdehyde;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Yang, CS Taichung Vet Gen Hosp, Dept Educ & Res, Taichung 407, Taiwan Taichung Vet Gen Hosp Taichung Taiwan 407 Taichung 407, Taiwan
Citazione:
C.S. Yang et al., "On-line, continuous and automatic monitoring of extracellular malondialdehyde concentration in anesthetized rat brain cortex", J CHROMAT B, 752(1), 2001, pp. 33-38

Abstract

An assay for in vivo, continuous and automatic monitoring of extracellularmalondialdehyde concentrations in anesthetized rat brain cortex was developed. This method involved the use of microdialysis perfusion, on-line derivatization and on-line high-performance liquid chromatographic analysis. Microdialysate from an implanted microdialysis probe was on-line reacted with thiobarbituric acid at 80 degreesC for 10 min prior to on-line collection and automatic injection into a HPLC system equipped with a fluorescence detector. This method gave a linear response between the concentrations of the malondialdehyde in the microdialysates and the TEP solution where the microdialysis probe was placed. This method was used to observe the increased extracellular malondialdehyde production following elevated extracellular glutamate levels, which were achieved by perfusion of L-trans-pyrrolidine-2,4-dicarboxylate, a competitive inhibitor of glutamate uptake transporter. (C)2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 15:24:15