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Titolo:
Determination of salicylate, gentisic acid and salicyluric acid in human urine by capillary electrophoresis with laser-induced fluorescence detection
Autore:
Zaugg, S; Zhang, X; Sweedler, J; Thormann, W;
Indirizzi:
Univ Bern, Dept Clin Pharmacol, CH-3010 Bern, Switzerland Univ Bern BernSwitzerland CH-3010 Pharmacol, CH-3010 Bern, Switzerland Univ Illinois, Dept Chem, Urbana, IL 61801 USA Univ Illinois Urbana IL USA 61801 linois, Dept Chem, Urbana, IL 61801 USA
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 752, anno: 2001,
pagine: 17 - 31
SICI:
1387-2273(20010305)752:1<17:DOSGAA>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACETYLSALICYLIC-ACID; HUMAN PLASMA; ZONE-ELECTROPHORESIS; SAMPLE PRETREATMENT; MAJOR METABOLITES; MASS-SPECTROMETRY; BODY-FLUIDS; HUMAN SERUM; CHROMATOGRAPHY; IMMUNOASSAYS;
Keywords:
salicylate; gentisic acid; salicyluric acid;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Thormann, W Univ Bern, Dept Clin Pharmacol, Murtenstr 35, CH-3010 Bern, Switzerland Univ Bern Murtenstr 35 Bern Switzerland CH-3010 , Switzerland
Citazione:
S. Zaugg et al., "Determination of salicylate, gentisic acid and salicyluric acid in human urine by capillary electrophoresis with laser-induced fluorescence detection", J CHROMAT B, 752(1), 2001, pp. 17-31

Abstract

Acetylsalicylic acid (Aspirin) is rapidly metabolized to salicylic acid (salicylate) and other compounds, including gentisic acid and salicyluric acid. Monitoring of salicylate and its metabolites is of toxicological, pharmacological and biomedical interest. Three capillary electrophoresis (CE) methods featuring alkaline aqueous buffers, laser-induced fluorescence (LIF) detection and no solute extraction or derivatization have been explored. A competitive binding, electrokinetic capillary-based immunoassay is developedthat recognizes the presence of salicylate and gentisic acid in urine. Differentiation of the two compounds, however, is problematic. With appropriate ultraviolet excitation, many salicylate-related compounds are fluorescentso that CE with direct urine injection and LIF detection permits the determination of salicylate, gentisic acid and salicyluric acid. Using a HeCd laser with 325 nm produces interference-free monitoring of all three compounds. Using 257 nm excitation from a frequency doubled Ar ion laser, native fluorescence of an endogenous urinary compound that co-migrates with gentisicacid is observed, With wavelength-resolved fluorescence detection, however, the two substances are distinguished. Furthermore, this technique, with comparison to literature data, permits the putative assignment of several peaks to other salicylate metabolites, namely glucuronide conjugates of salicylate and salicyluric acid. All three CE-LIF techniques have been applied to toxicological patient urines and urines collected after ingestion of 500 mg acetylsalicylic acid. CE results compare favorably with those obtained by a commercial fluorescence polarization immunoassay and by a conventional photometric assay. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 11:53:00