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Titolo:
Disialoganglioside GD3 is selectively expressed by developing and mature human mast cells
Autore:
Ren, SL; Kambe, N; Du, ZM; Li, YL; Xia, HZ; Kambe, M; Bieberich, E; Pozez, A; Grimes, M; Yu, RK; Irani, AM; Schwartz, LB;
Indirizzi:
Virginia Commonwealth Univ, Dept Internal Med, Richmond, VA 23298 USA Virginia Commonwealth Univ Richmond VA USA 23298 , Richmond, VA 23298 USA Virginia Commonwealth Univ, Dept Biochem & Mol Biophys, Richmond, VA 23298USA Virginia Commonwealth Univ Richmond VA USA 23298 s, Richmond, VA 23298USA Virginia Commonwealth Univ, Dept Surg, Richmond, VA 23298 USA Virginia Commonwealth Univ Richmond VA USA 23298 , Richmond, VA 23298 USA Virginia Commonwealth Univ, Dept Pathol, Richmond, VA 23298 USA Virginia Commonwealth Univ Richmond VA USA 23298 , Richmond, VA 23298 USA Virginia Commonwealth Univ, Dept Pediat, Richmond, VA 23298 USA Virginia Commonwealth Univ Richmond VA USA 23298 , Richmond, VA 23298 USA
Titolo Testata:
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
fascicolo: 2, volume: 107, anno: 2001,
pagine: 322 - 330
SICI:
0091-6749(200102)107:2<322:DGISEB>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOUSE MONOCLONAL-ANTIBODY; BASOPHILIC LEUKEMIA-CELLS; HUMAN T-LYMPHOCYTES; PHASE-I TRIAL; GANGLIOSIDE EXPRESSION; SIGNAL-TRANSDUCTION; DIFFERENTIATION; MELANOMA; TRYPTASE; CHYMASE;
Keywords:
GD3; mast cells; tryptase; Kit;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Schwartz, LB Virginia Commonwealth Univ, Dept Internal Med, POB 980263, Richmond, VA 23298 USA Virginia Commonwealth Univ POB 980263 Richmond VA USA 23298 A
Citazione:
S.L. Ren et al., "Disialoganglioside GD3 is selectively expressed by developing and mature human mast cells", J ALLERG CL, 107(2), 2001, pp. 322-330

Abstract

Background: Disialoganggioside GD3 is expressed on the surface of selectedcell types, Anti-GD3 mAb administered to human subjects with malignant melanoma produces signs and symptoms of immediate hypersensitivity reactions. Objective: The expression of GD3 by human mast cells was assessed during mast cell development in vitro and in samples of lung and skin. Methods: GD3 on tissue- and in vitro-derived mast cells was analyzed afterdouble labeling of cells for tryptase (G3 mAb) or Kit (YB5.B8 mAb) and GD3(R24 mAb). Glycolipids in extracts of fetal liver-derived mast cells were examined by using high-performance thin-layer chromatography. Results: Flow cytometry showed that the percentage of GD3(+) cells increased in parallel to Kit(+) cells during the recombinant human stem cell factor-dependent development of fetal liver-derived mast cells. Double-labeling experiments showed that GD3(+) cells were also surface Kit(+) and granule tryptase positive, identifying them as mast cells in preparations of lung-, skin-, fetal liver-, and cord blood-derived cells. The major acidic glycolipid detected was NeuAc alpha2-8NeuAc alpha2-3Gal beta1-4Glc beta1-1'Cer (GD3). Among peripheral blood leukocytes, only basophils and about 10% of the T cells were labeled with anti-GD3 mAb. Anti-GD3 mAb-conjugated magnetic beads were used to purify mast cells to greater than 90% purity from dispersed skin cells enriched to approximately 12% purity by means of density-dependent sedimentation but were less proficient for dispersed human lung mast cells, most likely because of other cell types that express GD3. Conclusion: GD3 is expressed on the surface of developing human mast cellsin parallel to tryptase in secretory granules and, like Kit, can serve as a target for their enrichment by immunoaffinity techniques.

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Documento generato il 29/11/20 alle ore 00:31:48