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Titolo:
Human mouse mast cell protease 7-like tryptase genes are pseudogenes
Autore:
Min, HK; Kambe, N; Schwartz, LB;
Indirizzi:
Virginia Commonwealth Univ, Dept Internal Med, Richmond, VA 23298 USA Virginia Commonwealth Univ Richmond VA USA 23298 , Richmond, VA 23298 USA
Titolo Testata:
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
fascicolo: 2, volume: 107, anno: 2001,
pagine: 315 - 321
SICI:
0091-6749(200102)107:2<315:HMMCP7>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
FETAL LIVER-CELLS; FC-EPSILON-RI; RECOMBINANT HUMAN; MONOCLONAL-ANTIBODIES; SYSTEMIC-ANAPHYLAXIS; COMPLEMENTARY-DNA; ACTIVATION; CHYMASE; BLOOD; ALPHA;
Keywords:
mast cell; RT-PCR; pseudogene tryptase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Schwartz, LB Virginia Commonwealth Univ, Dept Internal Med, POB 980263, Richmond, VA 23298 USA Virginia Commonwealth Univ POB 980263 Richmond VA USA 23298 A
Citazione:
H.K. Min et al., "Human mouse mast cell protease 7-like tryptase genes are pseudogenes", J ALLERG CL, 107(2), 2001, pp. 315-321

Abstract

Background: alpha -Tryptase and beta -tryptase are important clinical markers for mast cell-dependent disorders. A third family of tryptase genes on human chromosome 16 has been identified and called human mouse mast cell protease 7 (hmMCP-7)-like tryptase. Objective: This study was designed to determine whether these tryptase genes are expressed by human mast cells. Methods: A 2842-bp hmMCP-7-like tryptase gene was cloned and sequenced from a human placental genomic library. PCR and RT-PCR procedures, respectively, were used to determine whether this tryptase gene family was present in most genomes and whether it was expressed. Results: The tryptase clone was almost identical to the hmMCP-7-like tryptase II and I genes, and therefore it was called hmMCP-7-like tryptase III. All such genes encode a Gln(-3) like alpha -tryptase. They also terminate translation after amino acid 235, whereas alpha- and beta -tryptase genes each encode a 275-amino acid protein. In this study, cell lines HMC-1, KU812,and Mono-Mac-6; mast cells derived in vitro from cord blood and fetal liver progenitors; and mast cell-enriched preparations of dispersed skin and lung cells contained hmMCP-7-like tryptases in their genomes by PCR with gene-specific primers. To identify whether such genes were transcriptionally active, RT-PCR revealed alpha- or beta -tryptase products in all mast cell preparations and cell lines and in activated skin-derived mast cells, but no hmMCP-7-like tryptase products. Conclusion: These results indicate hmMCP-7-like tryptase (I, II, III) genes are pseudogenes and unlikely to affect measurements of alpha- and beta -tryptases.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 21:06:27