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Titolo:
Histamine alters gene expression in cultured human nasal epithelial cells
Autore:
Takeuchi, K; Kishioka, C; Ishinaga, H; Sakakura, Y; Majima, Y;
Indirizzi:
Mie Univ, Sch Med, Dept Otorhinolaryngol, Tsu, Mie 514, Japan Mie Univ Tsu Mie Japan 514 ed, Dept Otorhinolaryngol, Tsu, Mie 514, Japan Kaisei Hosp, Dept Otorhinolaryngol, Mie, Japan Kaisei Hosp Mie JapanKaisei Hosp, Dept Otorhinolaryngol, Mie, Japan
Titolo Testata:
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
fascicolo: 2, volume: 107, anno: 2001,
pagine: 310 - 314
SICI:
0091-6749(200102)107:2<310:HAGEIC>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; RECEPTOR MESSENGER-RNA; TIGHT-JUNCTION; ENDOTHELIAL-CELLS; FACTOR-ALPHA; ALLERGIC RHINITIS; ZONULA-OCCLUDENS; RELEASE; MUCOSA; ZO-1;
Keywords:
histamine receptors; mRNA; allergic rhinitis; reverse transcription-polymerase chain reaction; nasal mucosa; ZO-1; IL-8;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Majima, Y Mie Univ, Sch Med, Dept Otorhinolaryngol, 2-174 Edobashi, Tsu, Mie 514, Japan Mie Univ 2-174 Edobashi Tsu Mie Japan 514 i, Tsu, Mie 514, Japan
Citazione:
K. Takeuchi et al., "Histamine alters gene expression in cultured human nasal epithelial cells", J ALLERG CL, 107(2), 2001, pp. 310-314

Abstract

Background: Airway epithelial cells produce cytokines and participate in the regulation of mucosal immunity. Although nasal epithelial cells express histamine receptors, it is not exactly known how nasal epithelial cells respond to histamine. Objective: The objective of this study was to examine whether histamine can alter the expression of the 4 genes encoding H-1 receptor, IL-8, TNF-alpha and ZO-1 tight-junction protein in cultured nasal epithelial cells. Methods: We added histamine or vehicle to cultured human nasal epithelial cells and extracted RNA from them 4 hours later. After DNase treatment, mRNAs of beta -actin, H-1 receptor, IL-8, TNF-alpha, and ZO-1 tight-junction protein were amplified by using RT-PCR. Results: Histamine significantly upregulated IL-8 mRNA expression and significantly downregulated ZO-1 mRNA expression. The latter effect was blockedby pretreatment with mepyramine, an H-1 receptor antagonist. Conclusion: The reduction of ZO-1 mRNA by histamine may cause increased permeability of the mucosa during allergic reactions in the nose.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/20 alle ore 14:45:00