Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Identification of IgE-binding components of citrus red mite in sera of patients with citrus red mite-induced asthma
Autore:
Kim, HY; Park, HS; Kim, YK; Son, JW; Kim, HA; Suh, JH; Nahm, DH; Cho, SH; Min, KU; Kim, YY;
Indirizzi:
Ajou Univ, Sch Med, Dept Allergy & Clin Immunol, Suwon 442749, South KoreaAjou Univ Suwon South Korea 442749 in Immunol, Suwon 442749, South Korea Seoul Natl Univ, Dept Med, Seoul, South Korea Seoul Natl Univ Seoul South Korea tl Univ, Dept Med, Seoul, South Korea
Titolo Testata:
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
fascicolo: 2, volume: 107, anno: 2001,
pagine: 244 - 248
SICI:
0091-6749(200102)107:2<244:IOICOC>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
PANONYCHUS-CITRI; HOUSE DUST; ALLERGEN; FARINAE; FARMERS;
Keywords:
citrus red mite; IgE-binding component; major allergen; amino acid sequencing;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
11
Recensione:
Indirizzi per estratti:
Indirizzo: Park, HS Ajou Univ, Sch Med, Dept Allergy & Clin Immunol, Paldalgu Wonchondong San-5, Suwon 442749, South Korea Ajou Univ Paldalgu Wonchondong San-5 Suwon South Korea 442749 ea
Citazione:
H.Y. Kim et al., "Identification of IgE-binding components of citrus red mite in sera of patients with citrus red mite-induced asthma", J ALLERG CL, 107(2), 2001, pp. 244-248

Abstract

Background: Our previous investigations demonstrated that citrus red mite (CRM) antigen could cause IgE-mediated bronchoconstriction in exposed farmers working on citrus farms. Objective: This study was performed to confirm IgE-binding components and major allergens within the CRM antigens. Methods: Ten subjects who had been diagnosed as having CRM-induced asthma were enrolled. Serum-specific IgE antibodies to CRM antigens were measured by using an ELISA. To identify IgE-binding components and major allergens, SDS-PAGE, 2-dimensional PAGE, IgE-immunoblot analysis, and amino acid sequencing of major allergens were performed. Results: All the asthmatic subjects had high specific IgE antibodies to CRMs. Twelve percent SDS-PAGE analysis showed more than 10 protein bands ranging from 6 to 64 kd. SDS-PAGE and IgE-immunoblot analysis with each individual serum showed 5 IgE-binding components (11, 24, 35, 40, and 64 kd), with2 (24 and 35 kd) of them bound in more than 50% of the study subjects. Two-dimensional PAGE and IgE-immunoblot analysis demonstrated that the major allergen at 24 kd had 2 bands with different isoelectric points of 1.75 and 5.1. Thirty-five kilodaltons had one band with an isoelectric point of 4.75. All amino acid sequencing of the 2 major allergens was performed, which was not homologous with any previously characterized allergens. Conclusion: Five IgE-binding components and 2 major allergens (24 and 35 kd) were identified within the CRM antigen. The N-terminal amino acid sequence of the 2 major allergens (24 and 35 kd) was determined.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 11:58:16