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Titolo:
Screening effect of PEG on avidin binding to liposome surface receptors
Autore:
Kaasgaard, T; Mouritsen, OG; Jorgensen, K;
Indirizzi:
Tech Univ Denmark, Dept Chem, DK-2800 Lyngby, Denmark Tech Univ Denmark Lyngby Denmark DK-2800 t Chem, DK-2800 Lyngby, Denmark Royal Danish Sch Pharm, Dept Pharmaceut, DK-2100 Copenhagen, Denmark RoyalDanish Sch Pharm Copenhagen Denmark DK-2100 00 Copenhagen, Denmark
Titolo Testata:
INTERNATIONAL JOURNAL OF PHARMACEUTICS
fascicolo: 1-2, volume: 214, anno: 2001,
pagine: 63 - 65
SICI:
0378-5173(20010219)214:1-2<63:SEOPOA>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLY(ETHYLENE GLYCOL); BIOTIN; BILAYERS; INVIVO;
Keywords:
PEG liposomes; steric stabilization; receptor-ligand interaction; lipopolymer; drug targeting; avidin; biotin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Jorgensen, K Tech Univ Denmark, Dept Chem, DK-2800 Lyngby, Denmark Tech Univ Denmark Lyngby Denmark DK-2800 00 Lyngby, Denmark
Citazione:
T. Kaasgaard et al., "Screening effect of PEG on avidin binding to liposome surface receptors", INT J PHARM, 214(1-2), 2001, pp. 63-65

Abstract

This study investigates the screening effect of poly(ethylene glycol)-phospholipids (PE-PEG) on the interaction of avidin with PEGylated liposomes containing surface-bound biotin ligands. The influence of grafting density and lipopolymer chain length is examined. A simple fluorescence assay involving a receptor-mediated fluorescence increase of BODIPY-labeled avidin upon binding to biotinylated lipids is employed to study the screening effect ofsubmicellar concentrations of 1,2-dipalmitoyl-sn-glycero-3-phosphatidylethanolamine-N-[poly(ethylene glycol)-2000] (PE-PEG(2000)) and 1.2-dipalmitoyl-sn-glycero-3-phosphatidylethanolamine N-[poly(ethylene glycol)-5000] (PE-PEG(5000)) incorporated into 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) liposomes. The results show that incorporation of lipopolymers into DPPC lipid bilayers reduces binding of avidin to the biotinylated liposomes. and it is found that the screening effect of PE-PEG(5000) is stronger than that for PE-PEG(2000). Thus, the results reveal that both the grafting density and the polymer length of the PE-PEC lipopolymers are of importancefor the ability of water-soluble macromolecules to reach the surface of PEG liposomes. Furthermore. it is found that none of the lipopolymers completely prevents avidin from reaching the surface-bound biotin ligands. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 00:19:05