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Titolo:
Effects of docetaxel in combination with radiation on human head and neck cancer cells (ZMK-1) and cervical squamous cell carcinoma cells (CaSki)
Autore:
Pradier, O; Rave-Frank, M; Lehmann, J; Lucke, E; Boghun, O; Hess, CF; Schmidberger, H;
Indirizzi:
Univ Gottingen, Dept Radiotherapy & Radiat Oncol, D-37075 Gottingen, Germany Univ Gottingen Gottingen Germany D-37075 col, D-37075 Gottingen, Germany Stanford Univ, Sch Med, Dept Radiat Oncol, Stanford, CA 94305 USA StanfordUniv Stanford CA USA 94305 Radiat Oncol, Stanford, CA 94305 USA
Titolo Testata:
INTERNATIONAL JOURNAL OF CANCER
fascicolo: 6, volume: 91, anno: 2001,
pagine: 840 - 845
SICI:
0020-7136(20010315)91:6<840:EODICW>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN TUMOR; IN-VITRO; TAXOTERE; TAXOL; PACLITAXEL; LINES; APOPTOSIS; CISPLATIN; HELA;
Keywords:
radiotherapy; docetaxel; radiosensitisation; SCC-cell lines;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Pradier, O Univ Gottingen, Dept Radiotherapy & Radiat Oncol, Robert Koch Str 40, D-37075 Gottingen, Germany Univ Gottingen Robert Koch Str 40 Gottingen Germany D-37075 ny
Citazione:
O. Pradier et al., "Effects of docetaxel in combination with radiation on human head and neck cancer cells (ZMK-1) and cervical squamous cell carcinoma cells (CaSki)", INT J CANC, 91(6), 2001, pp. 840-845

Abstract

The purpose of this study was to determine, as we did for paclitaxel, the cytotoxic and radiosensitizing potential of docetaxel in human head and neck cancer cells (ZMK-I), and in cervical squamous cell carcinoma cells (CaSki). ZMK-I cells were incubated with docetaxel for 3, 9 or 24 hr before irradiation and 24 hr after irradiation. CaSki cells were incubated with docetaxel 24 hr before and after irradiation. For ZMK-I cells, the docetaxel concentrations (0.7, 0.7 and 0.35 nM) were determined to obtain approximately equivalent cell survival at the different incubation times (3, 9 and 24 hr, respectively). For CaSki cells, the necessary concentration of docetaxel was 0.07 nM. Radiation doses were given from 0 to 7 Gy, Cell survival was measured by a standard clonogenic assay after a 9-day incubation. Flow cytometry was used to measure the capacity of docetaxel to accumulate cells in theG2/M phase of the cell cycle. We observed a weak accumulation of cells in the G2/M phase for the ZMK-I cells and a pronounced accumulation for CaSki cells. For docetaxel incubation before irradiation, the isoeffect enhancement ratios for ZMK-I cells determined at the 37% survival level were 1.18, 2.01, and 2.40 for pre-incubation at 3, 9 and 24 hr, respectively; for CaSkicells the ratio was 1.44. For a docetaxel incubation of 24 hr after irradiation, the isoeffect enhancement ratios determined at the 37% survival level were 1.54 and 1.17 for the ZMK-I, and CaSki cells, respectively. A radiosensitizing effect of docetaxel could be demonstrated unambiguously in the two cell lines used. In contrast to our previously published results with paclitaxel, docetaxel seems to be a better radiosensitizer than paclitaxel. (C) 2001 Wiley-Liss. Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 22:53:34