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Titolo:
Characterization of I-123-vascular endothelial growth factor-binding sitesexpressed on human tumour cells: Possible implication for tumour scintigraphy
Autore:
Li, SR; Peck-Radosavljevic, M; Koller, E; Koller, F; Kaserer, K; Kreil, A; Kapiotis, S; Hamwi, A; Weich, HA; Valent, P; Angelberger, P; Dudczak, R; Virgolini, I;
Indirizzi:
Univ Vienna, Dept Nucl Med, AKH, A-1090 Vienna, Austria Univ Vienna Vienna Austria A-1090 Nucl Med, AKH, A-1090 Vienna, Austria Univ Vienna, Dept Gastroenterol & Hepatol, Vienna, Austria Univ Vienna Vienna Austria ept Gastroenterol & Hepatol, Vienna, Austria Univ Vienna, Dept Physiol, Vienna, Austria Univ Vienna Vienna AustriaUniv Vienna, Dept Physiol, Vienna, Austria Univ Vienna, Dept Clin Pathol, Vienna, Austria Univ Vienna Vienna Austria iv Vienna, Dept Clin Pathol, Vienna, Austria Univ Vienna, Dept Lab Med, Vienna, Austria Univ Vienna Vienna AustriaUniv Vienna, Dept Lab Med, Vienna, Austria Univ Vienna, Inst Biochem & Mol Cell Biol, Vienna, Austria Univ Vienna Vienna Austria nst Biochem & Mol Cell Biol, Vienna, Austria Univ Vienna, Dept Internal Med 1, Div Haematol & Haemostaseol, Vienna, Austria Univ Vienna Vienna Austria Div Haematol & Haemostaseol, Vienna, Austria Soc Biotechnol Res, Dept Gene Express, Braunschweig, Germany Soc Biotechnol Res Braunschweig Germany Express, Braunschweig, Germany Ludwig Boltzmann Inst Nucl Med, Vienna, Austria Ludwig Boltzmann Inst NuclMed Vienna Austria Nucl Med, Vienna, Austria Res Ctr Seibersdorf, Seibersdorf, Austria Res Ctr Seibersdorf Seibersdorf Austria ibersdorf, Seibersdorf, Austria
Titolo Testata:
INTERNATIONAL JOURNAL OF CANCER
fascicolo: 6, volume: 91, anno: 2001,
pagine: 789 - 796
SICI:
0020-7136(20010315)91:6<789:COIEGF>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-MELANOMA CELLS; FACTOR RECEPTORS; TYROSINE KINASE; VEGF RECEPTORS; ANGIOGENESIS; KDR; VEGF(121); DISEASE; INVIVO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Li, SR Univ Vienna, Dept Nucl Med, AKH, Ebene 3L,Waehringer Guertel 18-20,A-1090Vienna, Austria Univ Vienna Ebene 3L,Waehringer Guertel 18-20 Vienna Austria A-1090
Citazione:
S.R. Li et al., "Characterization of I-123-vascular endothelial growth factor-binding sitesexpressed on human tumour cells: Possible implication for tumour scintigraphy", INT J CANC, 91(6), 2001, pp. 789-796

Abstract

To explore the possibility of vascular endothelial growth factor (VEGF) receptor scintigraphy of primary tumours and their metastases, we analysed the binding properties of (123)[labelled VEGF(165) (I-123-VEGF(165)) and I-123-VEGF(121) to human umbilical vein endothelial cells (HUVECs), several human tumour cell lines (HMC-1, A43I, KU812, U937, HEP-I, HEP-G2, HEP-3B and Raji), a variety of primary human tumours (n = 40) and some adjacent non-neoplastic tissues as well as normal human peripheral blood cells in vitro. Two classes of high-affinity I-123-VEGF(165)-binding site were found on the cell surface of HUVECs. In contrast, one class of high-affinity binding sites for I-123-VEGF(165) was found on HMC-I, A43I, HEP-I, HEP-GZ, HEP-3B and U937 cells as well as many primary tumours. For I-123-VEGF(121), a Single class of high-affinity binding site was found on certain cell lines (HUVEC, HEP-I and HMC-I) and distinct primary tumours (primary melanomas, ductal breast cancers and ovarian carcinomas as well as meningiomas). Tumour cells expressed significantly higher numbers of VECF receptors compared with normalperipheral blood cells and adjacent non-neoplastic tissues. Immunohistochemical staining revealed that the VEGF receptor Flk-I is expressed to a muchhigher extent within malignant tissues compared with neighbouring non-neoplastic cells. We observed significantly greater specific binding of I-123-VEGF(165) and I-123-VEGF(121) to a variety of human tumour cells/tissues compared with the corresponding normal tissues or normal peripheral blood cells. In comparison with I-123-VEGF(121), I-123-VEGF(121) bound to a higher number of different tumour cell types with a higher capacity. Thus, I-123-VEGF(165) may be a potentially useful tracer for in vivo imaging of solid rumours. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 21:53:49