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Titolo:
Released ATP is an extracellular cytotoxic mediator in salivary histatin 5-induced killing of Candida albicans
Autore:
Koshlukova, SE; Araujo, MWB; Baev, D; Edgerton, M;
Indirizzi:
SUNY Buffalo, Dept Oral Biol, Sch Dent Med, Buffalo, NY 14214 USA SUNY Buffalo Buffalo NY USA 14214 ol, Sch Dent Med, Buffalo, NY 14214 USA SUNY Buffalo, Dept Restorat Dent, Sch Dent Med, Buffalo, NY 14214 USA SUNYBuffalo Buffalo NY USA 14214 nt, Sch Dent Med, Buffalo, NY 14214 USA
Titolo Testata:
INFECTION AND IMMUNITY
fascicolo: 12, volume: 68, anno: 2000,
pagine: 6848 - 6856
SICI:
0019-9567(200012)68:12<6848:RAIAEC>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
SACCHAROMYCES-CEREVISIAE; INNATE IMMUNITY; CELL-VOLUME; RECEPTOR; YEAST; MECHANISM; CLONING; P2X(7); PURINOCEPTORS; FERMENTATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Edgerton, M SUNY Buffalo, Dept Oral Biol, Sch Dent Med, 310 Foster Hall,Main St Campus,3435 Main St, Buffalo, NY 14214 USA SUNY Buffalo 310 Foster Hall,Main St Campus,3435 Main St Buffalo NY USA 14214
Citazione:
S.E. Koshlukova et al., "Released ATP is an extracellular cytotoxic mediator in salivary histatin 5-induced killing of Candida albicans", INFEC IMMUN, 68(12), 2000, pp. 6848-6856

Abstract

Salivary histatins (Hsts) are antifungal peptides with promise as therapeutic agents against candidiasis. Hst 5 kills the fungal pathogen Candida albicans via a mechanism that involves release of cellular ATP in the absence of cytolysis. Here we demonstrate that released ATP has a further role in Hst 5 killing. Incubation of the cells with ATP analogues induced cell death, and addition of the ATP scavenger apyrase to remove extracellular ATP released during Hst 5 treatment resulted in a reduction in cell killing. Experiments using anaerobically grown C. albicans with decreased susceptibility to Hst 5 confirmed that depletion of cellular ATP as a result of ATP effluxwas not sufficient to cause cell death. In contrast to Hst-susceptible aerobic cultures, anaerobically grown cells were not killed by exogenously applied ATP. These findings established that Hst binding, subsequent entry into the cells, and ATP release precede the signal for cytotoxicity, which is mediated by extracellular ATP. In a higher-eukaryote paradigm, released ATPacts as a cytotoxic mediator by binding to membrane nucleotide P2X receptors. Based on a pharmacological profile and detection of a C. albicans 60 kDa membrane protein immunoreactive with antibody to P2X(7) receptor, we propose that released ATP in response to Hst 5 activates candidal P2X(7)-like receptors to cause cell death.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 19:36:34