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Titolo:
Reduced fimbria-associated activities of Porphyromonas gingivalis induced by recombinant fimbrial expression
Autore:
Takahashi, Y; Yoshimoto, H; Kato, D; Hamada, N; Arai, M; Umemoto, T;
Indirizzi:
Kanagawa Dent Coll, Dept Oral Microbiol, Yokosuka, Kanagawa 2388580, JapanKanagawa Dent Coll Yokosuka Kanagawa Japan 2388580 anagawa 2388580, Japan
Titolo Testata:
FEMS MICROBIOLOGY LETTERS
fascicolo: 2, volume: 195, anno: 2001,
pagine: 217 - 222
SICI:
0378-1097(20010220)195:2<217:RFAOPG>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
BACTEROIDES-GINGIVALIS; MOLECULAR-CLONING; GENES DOWNSTREAM; PROTEINS; FIMA; BINDING; TRANSFORMATION; CONSTRUCTION; SEQUENCE;
Keywords:
transformation; fimbria; adhesion; Porphyromonas gingivalis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Umemoto, T Kanagawa Dent Coll, Dept Oral Microbiol, 82 Inaoka Cho, Yokosuka, Kanagawa2388580, Japan Kanagawa Dent Coll 82 Inaoka Cho Yokosuka Kanagawa Japan 2388580
Citazione:
Y. Takahashi et al., "Reduced fimbria-associated activities of Porphyromonas gingivalis induced by recombinant fimbrial expression", FEMS MICROB, 195(2), 2001, pp. 217-222

Abstract

The adhesion properties of the recombinant fimbriae (r-fimbriae) recoveredfrom a YH522 transformant of Porphyromonas gingivalis which harbors a chimeric plasmid, pYHF2, containing the fimA gene of strain 381 were compared with those of the endogenous fimA Fimbriae of strain 33277. The adhesion level of the r-fimbriae to Actinomyces viscosus was clearly lower than that ofthe endogenous fimbriae. In addition, the r-fimbriae were shown to lack some minor components detectable in the endogenous fimbriae. The plasmid pYHF2 prepared from the YH522 transformant was then transformed into six different P. gingivalis strains and the resultant pYHF2-containing strains were examined for their fimbrial expression. In spite of the presence of a considerable diversity in the expression level of the r-fimbriae among these transformants, it was evident that the strains expressing higher levels of the r-fimbriae exhibited a greater decrease in adhesion activity to other bacteria and to oral epithelial cells, as well as in self-aggregation. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 01:35:09