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Titolo:
Differential regulation of trkA and p75 in noradrenergic pelvic autonomic ganglion cells after deafferentation of their cholinergic neighbours
Autore:
Keast, JR; Kepper, ME;
Indirizzi:
Prince Wales Med Res Inst, Sydney, NSW 2031, Australia Prince Wales Med Res Inst Sydney NSW Australia 2031 , NSW 2031, Australia Univ Queensland, Dept Physiol & Pharmacol, Brisbane, Qld 4072, Australia Univ Queensland Brisbane Qld Australia 4072 Brisbane, Qld 4072, Australia
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 2, volume: 13, anno: 2001,
pagine: 211 - 220
SICI:
0953-816X(200101)13:2<211:DROTAP>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
NERVE GROWTH-FACTOR; AFFINITY NEUROTROPHIN RECEPTOR; MATURE SYMPATHETIC NEURONS; DORSAL-ROOT GANGLIA; RAT SPINAL-CORD; MALE SEX-ORGANS; URINARY-BLADDER; ADULT-RAT; PREGANGLIONIC NEURONS; SENSORY NEURONS;
Keywords:
preganglionic; rat; spinal nerve; sprouting; urogenital;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Keast, JR Prince Wales Med Res Inst, Barker St, Randwick, NSW 2031, Australia Prince Wales Med Res Inst Barker St Randwick NSW Australia 2031
Citazione:
J.R. Keast e M.E. Kepper, "Differential regulation of trkA and p75 in noradrenergic pelvic autonomic ganglion cells after deafferentation of their cholinergic neighbours", EUR J NEURO, 13(2), 2001, pp. 211-220

Abstract

In rats, following lesion of lumbar or sacral preganglionic axons, many pelvic ganglion cells undergo axogenesis to form baskets of terminals around select populations of nearby ganglion cells. The aim of the current study was to address mechanisms underlying initiation of this sprouting, focusing on a possible role for nerve growth factor (NGF). Immunohistochemical localization of NGF receptors (trkA and p75) showed that virtually all noradrenergic and a minority of cholinergic pelvic neurons expressed both receptors. Terminals immunoreactive for each substance were found in pelvic viscera. In pelvic ganglia, many glial cells expressed p75 but not trkA, and very few lumbar or sacral preganglionic neurons expressed either receptor. Lumbar and/or sacral preganglionic inputs were removed from ganglion cells by cutting the hypogastric, pelvic or both nerves, and tissues analysed 8 days later. Levels of receptor expression in noradrenergic pelvic ganglion cells were estimated by calculating the proportion that were receptor-immunopositive, and quantifying the intensity of trkA or p75 immunofluorescence. No lesion had a significant effect on trkA expression, however, a marked decrease in p75 occurred after cutting pelvic nerves, i.e. after deafferentation of neighbouring cholinergic neurons. These injuries appeared to cause little overall change in glial p75 expression. This study shows that manipulations that trigger sprouting from noradrenergic pelvic neurons cause downregulation of p75 but not trkA. Interestingly, this is occurring while some of their target organs are synthesizing high levels of NGF. This contrasts with other NGF-sensitive cells, in which one or both receptor types are upregulated by increased exposure to the ligand. The current study is also the first to show a change in p75 expression in neurons that are neither deafferentednor axotomized.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 00:47:06