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Titolo:
Retroviral infection of the FGF2 gene into MCF-7 cells induces branching morphogenesis, retards cell growth and suppresses tumorigenicity in nude mice
Autore:
Liu, D; Buluwela, L; All, S; Thomson, S; Gomm, JJ; Coombes, RC;
Indirizzi:
Imperial Coll Sch Med, Div Med, Dept Canc Med, London W12 0NN, England Imperial Coll Sch Med London England W12 0NN ed, London W12 0NN, England
Titolo Testata:
EUROPEAN JOURNAL OF CANCER
fascicolo: 2, volume: 37, anno: 2001,
pagine: 268 - 280
SICI:
0959-8049(200101)37:2<268:RIOTFG>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
BREAST-CANCER-CELLS; ESTROGEN-RECEPTOR-ALPHA; HUMAN PANCREATIC-CANCER; MAP KINASE ACTIVATION; CARCINOMA CELLS; MAMMARY-GLAND; IN-VITRO; EXPRESSION; LINE; INHIBITION;
Keywords:
basic fibroblast growth factor; breast cancer; morphogenesis; tumorigenicity; oestrogen receptor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Liu, D Univ London Univ Coll, Rayne Inst, 5 Univ St, London WC1E 6JJ, England Univ London Univ Coll 5 Univ St London England WC1E 6JJ J, England
Citazione:
D. Liu et al., "Retroviral infection of the FGF2 gene into MCF-7 cells induces branching morphogenesis, retards cell growth and suppresses tumorigenicity in nude mice", EUR J CANC, 37(2), 2001, pp. 268-280

Abstract

FGF2 (basic fibroblast growth factor) is a multifunctional growth factor and exhibits diverse function in different cell types. In breast, loss of FGF2 expression is associated with malignant progression. In order to understand the role of FGF2 in maintenance of normal breast structures and controlof cell growth, we restored FGF2 expression in the breast cancer cell lineMCF-7. The FGF2 retrovirally infected MCF-7 cells (MCF-7.F2.5) not only expressed FGF2 in cytoplasm and nuclei, but also released FGF2 into culture medium both on plastic and in Matrigel conditions. The MCF-7.F2.5 cells formed branches in Matrigel and this effect was abolished by the addition of a neutralising anti-FGF2 antibody or function blocking antibodies to alpha2, alpha3 and beta1 integrins. Furthermore, MCF-7.F2.5 cells lost their ability for anchorage-independent growth in soft agar. When MCF-7 and MCF-7.F2.5 cells were injected into nude mice, there was a 1.6- to 3.2-fold reduction of tumour volume with MCF-7.F2.5 cells in comparison with the parental MCF-7 cells. MCF-7.F2.5 cells also demonstrated a reduction in oestrogen receptor-alpha (ER alpha) bath. In vitro and in vivo. Our results suggest that introduction of the FGF2 gene into MCF-7 cells altered the malignant tumour cells towards a more benign phenotype bl vitro and in vivo. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/10/20 alle ore 00:18:44