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Titolo:
Age-related changes in energy production in fresh senescence-accelerated mouse brain slices as revealed by positron autoradiography
Autore:
Omata, N; Murata, T; Fujibayashi, Y; Waki, A; Sadato, N; Yoshimoto, M; Wada, Y; Yonekura, Y;
Indirizzi:
Fukui Med Univ, Dept Neuropsychiat, Fukui 9101193, Japan Fukui Med Univ Fukui Japan 9101193 t Neuropsychiat, Fukui 9101193, Japan Fukui Med Univ, Biomed Imaging Res Ctr, Fukui 9101193, Japan Fukui Med Univ Fukui Japan 9101193 Imaging Res Ctr, Fukui 9101193, Japan
Titolo Testata:
DEMENTIA AND GERIATRIC COGNITIVE DISORDERS
fascicolo: 2, volume: 12, anno: 2001,
pagine: 78 - 84
SICI:
1420-8008(200103/04)12:2<78:ACIEPI>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUCOSE-METABOLISM; MITOCHONDRIAL DYSFUNCTION; DYNAMIC CHANGES; ANIMAL-MODEL; MURINE MODEL; RAT; SAM; TRANSPORT; BLOOD;
Keywords:
brain slice; energy production; glucose metabolism; senescence-accelerated mouse; glycolytic capacity; mitochondrial function; O-2 deprivation; 2,4-dinitrophenol;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Murata, T Fukui Med Univ, Dept Neuropsychiat, Matsuoka Cho, Fukui 9101193,Japan Fukui Med Univ Matsuoka Cho Fukui Japan 9101193 9101193, Japan
Citazione:
N. Omata et al., "Age-related changes in energy production in fresh senescence-accelerated mouse brain slices as revealed by positron autoradiography", DEMENT G C, 12(2), 2001, pp. 78-84

Abstract

To investigate age-related changes in cerebral energy production, we compared senescence-accelerated prone mice (SAMP8) as an animal model of accelerated aging and senescence-accelerated resistant mice (SAMR1) as a control. Considering that the cerebral glucose metabolic rate (CMRglc) at the time of O-2 deprivation and 2,4-dinitrophenol (DNP) loading would reflect anaerobic glycolytic capacity and mitochondrial function, respectively, we investigated dynamic changes in CMRglc before and after loading with these perturbations. Fresh brain slices were incubated with [F-18]2-fluoro-2-deoxy-D-glucose ([F-18]FDG) in oxygenated Krebs-Ringer solution at 36 degreesC, and serial two-dimensional time-resolved images of [F-18]FDG uptake in these slices were obtained on the imaging plates. The fractional Fate constant (=k(3)*) of [F-18]FDG proportional to the CMRglc was evaluated by applying the Gjedde-Patlak graphical method to the image data. The k(3)* value before the hypoxic perturbation in all of the brain sites analyzed was higher in SAMP8than SAMR1 in both the 2- and 10-month-old groups. With O-2 deprivation, k(3)* values were higher without site specificity in the 2-month-old SAMP8 than in 2-month-old SAMR1, whereas in 10-month-old mice, there was no significant difference between the two groups. In contrast, with DNP loading, while no significant difference was noted between 2-month-old SAMP8 and 2-month-old SAMR1, in 10-month-old mice, the SAMP8 group showed lower values in certain regions than SAMR1 mice. These results suggest that in the brain tissue of SAMP8, a marked transient enhancement of anaerobic glycolytic capacity in the 2-month-olds and a decrease in mitochondrial function in the subsequent period occur, as a result of which glucose metabolism appears to be enhanced in both the 2- and 10-month-old groups compared to SAMR1 mice. Copyright (C) 2001 S. Karger AG, Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/01/20 alle ore 12:38:13