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Titolo:
Plug flow cytometry extends analytical capabilities in cell adhesion and receptor pharmacology
Autore:
Edwards, BS; Kuckuck, FW; Prossnitz, ER; Okun, A; Ransom, JT; Sklar, LA;
Indirizzi:
Univ New Mexico, Hlth Sci Ctr, Dept Pathol, Canc Res & Treatment Ctr, Albuquerque, NM 87131 USA Univ New Mexico Albuquerque NM USA 87131 t Ctr, Albuquerque, NM 87131 USA Univ New Mexico, Hlth Sci Ctr, Dept Cell Biol & Physiol, Canc Res & Treatment Ctr, Albuquerque, NM 87131 USA Univ New Mexico Albuquerque NM USA 87131 t Ctr, Albuquerque, NM 87131 USA Axiom Biotechnol Inc, San Diego, CA USA Axiom Biotechnol Inc San Diego CAUSA Biotechnol Inc, San Diego, CA USA Los Alamos Natl Labs, Los Alamos, NM USA Los Alamos Natl Labs Los Alamos NM USA mos Natl Labs, Los Alamos, NM USA
Titolo Testata:
CYTOMETRY
fascicolo: 3, volume: 43, anno: 2001,
pagine: 211 - 216
SICI:
0196-4763(20010301)43:3<211:PFCEAC>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
P-SELECTIN; NEUTROPHILS; KILLER; CA-2+;
Keywords:
flow cytometry; automation; sample handling; cell adhesion;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
14
Recensione:
Indirizzi per estratti:
Indirizzo: Edwards, BS Univ New Mexico, Hlth Sci Ctr, Dept Pathol, Canc Res & Treatment Ctr, CRF Room 217,2325 Camino Salud, Albuquerque, NM 87131 USA Univ New Mexico CRF Room 217,2325 Camino Salud Albuquerque NM USA 87131
Citazione:
B.S. Edwards et al., "Plug flow cytometry extends analytical capabilities in cell adhesion and receptor pharmacology", CYTOMETRY, 43(3), 2001, pp. 211-216

Abstract

Background: Plug flow cytometry is a recently developed system for the automated delivery of multiple small boluses or "plugs" of cells or particles to the flow cytometer for analysis. Important system features are that sample plugs are of precisely defined volume and that the sample vessel need not be pressurized. We describe how these features enable direct cell concentration determinations and novel ways to integrate flow cytometers with other analytical instruments. Methods: Adhesion assays employed human polymorphonuclear neutrophils (PMNs) loaded with Fura Red and Chinese hamster ovary (CHO) cells cotransfectedwith genes for green fluorescent protein (GFP) and human P-selectin. U937 cells expressing the human 7-transmembrane formyl peptide receptor were loaded with the fluorescent probe indo-1 for intracellular ionized calcium determinations. A computer-controlled syringe or peristaltic pump loaded the sample into a sample loop of the plug now coupler, a reciprocating eight-port valve. When the valve position was switched, the plug of sample in the sample loop was transported to the flow cytometer by a pressure-driven fluid line. Results: In stirred mixtures of PMNs and CHO cells, we used plug flow cytometry to directly quantify changes in concentrations of nonadherent singletPMNs. This approach enabled accurate quantification of adherent PMNs in multicell aggregates. We constructed a novel plug flow interface between the flow cytometer and a cone-plate viscometer to enable real-time flow cytometric analysis of cell-cell adhesion under conditions of uniform shear. The High Throughput Pharmacology System (HTPS) is an instrument used for automated programming of complex pharmacological cell treatment protocols. It was interfaced via the plug flow coupling device to enable rapid (<5 min) flow cytometric characterization of the intracellular calcium dose-response profile of U937 cells to formyl peptide. Conclusions: By facilitating the coupling of flow cytometers to other fluidics-based analytical instruments, plug flow cytometry has extended analytical capabilities in cell adhesion and pharmacological characterization of receptor-ligand interactions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 21:41:47