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Titolo:
Value of antigen detection using an enzyme immunoassay in the diagnosis and prediction of invasive aspergillosis in two adult and pediatric hematology units during a 4-year prospective study
Autore:
Sulahian, A; Boutboul, F; Ribaud, P; Leblanc, T; Lacroix, C; Derouin, F;
Indirizzi:
Hop St Louis, Lab Parasitol Mycol, F-75475 Paris 10, France Hop St Louis Paris France 10 b Parasitol Mycol, F-75475 Paris 10, France Hop St Louis, Unite Greffe de Moelle, F-75475 Paris, France Hop St Louis Paris France F-75475 reffe de Moelle, F-75475 Paris, France Hop St Louis, Serv Hematol Pediat, F-75475 Paris 10, France Hop St Louis Paris France 10 rv Hematol Pediat, F-75475 Paris 10, France
Titolo Testata:
CANCER
fascicolo: 2, volume: 91, anno: 2001,
pagine: 311 - 318
SICI:
0008-543X(20010115)91:2<311:VOADUA>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
LINKED-IMMUNOSORBENT-ASSAY; LATEX AGGLUTINATION-TEST; LIPOSOMAL AMPHOTERICIN-B; GALACTOMANNAN ANTIGEN; SERUM GALACTOMANNAN; INFECTION; FUMIGATUS;
Keywords:
invasive aspergillosis; circulating antigen; enzyme-linked immunosorbent assay; hematology; bone marrow transplantation; children;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Sulahian, A Hop St Louis, Lab Parasitol Mycol, 1 Ave Claude Vellefaux, F-75475 Paris 10, France Hop St Louis 1 Ave Claude Vellefaux Paris France 10 0, France
Citazione:
A. Sulahian et al., "Value of antigen detection using an enzyme immunoassay in the diagnosis and prediction of invasive aspergillosis in two adult and pediatric hematology units during a 4-year prospective study", CANCER, 91(2), 2001, pp. 311-318

Abstract

BACKGROUND. Invasive aspergillosis (LA) is a well recognized, life-threatening infection in neutropenic patients and stem cell transplantation recipients. Early diagnosis is important to achieve the best outcome for these patients; however, definite proof often is difficult to obtain due to counterindicated invasive procedures. METHODS. This study evaluated the specificity and sensitivity of the detection of galactomannan (GM) for the diagnostic and prediction of IA in 347 children from the Pediatric Hematology Service and 450 patients from the Bone Marrow Transplantation Unit at the Hopital Saint-Louis in Paris. Serial screening of Aspergillus GM circulating antigen was evaluated using a doublesandwich ELISA assay (Platelia Aspergillus) on 6209 sera. Among the patients studied, 53 presented with confirmed IA (n = 27 patients) or probable IA(n = 26 patients). RESULTS. Antigen was detected on at least two sequential sera in 48 of 53 patients, with a sensitivity of 90.6%. GM antigenemia was detected before the onset of radiologic signs in 31 of 48 patients (64.6%), with a mean of -8.4 days, and before clinical symptoms in 18 of 48 patients (39.6%), with amean of -6.9 days. In patients without IA, 44 of 744 had positive antigenemia, resulting in a specificity of 94%. False positive results could not berelated to the presence of a concurrent mucositis. CONCLUSIONS. This large, prospective study allowed the authors to define better the conditions for the use of GM immunocapture ELISA in surveying patients who are at high risk for IA. The presence of antigen has a good diagnostic value mainly when there is an increase in the titer on two consecutive sera samples. A repeated negative result is a strong argument against thediagnosis of IA; however, an awareness of the possibility of unexplained false negative results is important. (C) 2001 American Cancer Society.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 10:41:53