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Titolo:
Structural diversity of mast cell granules in black and white skin
Autore:
Sueki, H; Whitaker-Menezes, D; Kligman, AM;
Indirizzi:
Univ Penn, Sch Med, Dept Dermatol, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 Dermatol, Philadelphia, PA 19104 USA Thomas Jefferson Univ, Jefferson Med Coll, Dept Pathol Anat & Cell Biol, Philadelphia, PA 19107 USA Thomas Jefferson Univ Philadelphia PA USA 19107 hiladelphia, PA 19107 USA
Titolo Testata:
BRITISH JOURNAL OF DERMATOLOGY
fascicolo: 1, volume: 144, anno: 2001,
pagine: 85 - 93
SICI:
0007-0963(200101)144:1<85:SDOMCG>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
ULTRASTRUCTURAL ANALYSIS; SERINE PROTEINASES; HUMAN-T; RECOVERY; DEGRANULATION; SCLERODERMA; TRYPTASE; DISEASE;
Keywords:
cathepsin G; immunoelectron microscopy; mast cell granule; tryptase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Sueki, H Showa Univ, Sch Med, Dept Dermatol, Shinagawa Ku, 1-5-8 Hatanodai, Tokyo 1428666, Japan Showa Univ 1-5-8 Hatanodai Tokyo Japan 1428666 yo 1428666, Japan
Citazione:
H. Sueki et al., "Structural diversity of mast cell granules in black and white skin", BR J DERM, 144(1), 2001, pp. 85-93

Abstract

Background There are conflicting reports of structural differences betweenblack and white skin, other than pigmentary differences. Objectives To evaluate differences in mast cells between black and white skin. Methods Biopsies of normal buttock skin were obtained from four African-American males (29.2 +/- 3.0 years old) and four Caucasian males (29.4 +/- 1.2 years old) and processed routinely for electron microscopy. For the quantitative assessment of mast cell granules, five electron micrographs at a final magnification of x 53,700 were analysed for each individual, using a computer-assisted image analyser. More than 10 granules per cell, and a totalof 1210 granules, were evaluated for their internal structures. Results Mast cells in black skin contained larger granules than those in white skin (P < 0.0001). In black skin, fusion of granules seemed to accountfor the larger sizes. The percentage of granule matrix occupied by curved lamellae was higher in white skin, whereas parallel-linear striations were more frequent in black skin (P < 0.05). The subgranular distribution of themast cell proteases, tryptase and cathepsin G, were evaluated by immunoelectron microscopy. Tryptase reactivity was localized preferentially over theparallel-linear striations and partially over the dark amorphous subregions within granules of black skin, whereas it was confined to the peripheral area of granules, including curved lamellae, in white skin. Cathepsin G reactivity was more intense over the electron-dense amorphous areas in both groups, while parallel-linear striations in black skin and curved lamellae inwhite skin were negative. Conclusions This study has confirmed ultrastructural differences in mast cell granules between black and white skin, which may be of functional importance.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 08:52:17